Department of Dermatology, Geneva University Hospital, Geneva, Switzerland.
Exp Dermatol. 2012 Jan;21(1):19-24. doi: 10.1111/j.1600-0625.2011.01394.x. Epub 2011 Nov 15.
We assessed the ability of ebselen, a glutathione peroxidase mimic, to reduce pigmentation in various models. In murine B16 melanocytes, 25 μm ebselen inhibited melanogenesis and induced a depolymerisation of actin filaments. In co-cultures of B16 melanocytes with BDVII keratinocytes, a pretreatment of melanocytes with ebselen resulted in a strong inhibition of melanosome transfer to keratinocytes, as shown under optical and electron microscopy. In reconstructed epidermis, topical 0.5% ebselen led to a twofold decrease of melanin without affecting the density of active melanocytes. A similar result was obtained with topical 0.5% ebselen in black guinea pig ears. Ebselen induced a decrease of epidermal melanin parallel to a localisation of melanin and melanosomes in the basal layer. Ebselen appears as a new depigmenting compound that inhibits melanin synthesis and melanosome transfer to keratinocytes.
我们评估了谷胱甘肽过氧化物酶模拟物依布硒啉在各种模型中减少色素沉着的能力。在鼠 B16 黑素细胞中，25μm 的依布硒啉抑制黑素生成并诱导肌动蛋白丝解聚。在 B16 黑素细胞与 BDVII 角质形成细胞的共培养物中，依布硒啉预处理黑素细胞导致黑素小体向角质形成细胞的转移受到强烈抑制，在光学和电子显微镜下可见。在重建的表皮中，局部使用 0.5%的依布硒啉导致黑色素减少一倍，而不影响活性黑素细胞的密度。在黑豚鼠耳朵中局部使用 0.5%的依布硒啉也得到了类似的结果。依布硒啉诱导表皮黑色素减少，同时黑色素和黑素小体在基底层定位。依布硒啉是一种新的脱色化合物，可抑制黑色素合成和黑素小体向角质形成细胞的转移。
