Naughton Bart J, Thirtamara-Rajamani Keerthi, Wang Chuansong, During Matthew J, Gu Howard H
Department of Pharmacology, The Ohio State University College of Medicine, Columbus, Ohio, USA.
Neuroreport. 2012 Jan 4;23(1):1-5. doi: 10.1097/WNR.0b013e32834d2216.
Dopamine signaling in the nucleus accumbens is critical in mediating the effects of cocaine. There are two splice variants of dopamine D2 receptors, D2L and D2S, which are believed to have different functional roles. Here, we show, that knocking down D2L selectively using viral-mediated short-hairpin RNA led to a slight but significant decrease in basal locomotor activity with no significant change in cocaine-induced stimulation of locomotion. The knockdown appears to produce a trend of reduced conditioned place preference to cocaine but the difference was not statistically significant. Our results demonstrated that the splice variants of D2 receptors can be selectively manipulated in vivo in specific brain regions allowing more specific studies of each D2 receptor isoform.
伏隔核中的多巴胺信号传导在介导可卡因的作用方面至关重要。多巴胺D2受体有两种剪接变体，即D2L和D2S，人们认为它们具有不同的功能作用。在此，我们表明，使用病毒介导的短发夹RNA选择性敲低D2L会导致基础运动活性略有但显著降低，而可卡因诱导的运动刺激无显著变化。敲低似乎产生了对可卡因条件性位置偏好降低的趋势，但差异无统计学意义。我们的结果表明，D2受体的剪接变体可以在体内特定脑区进行选择性操纵，从而对每种D2受体亚型进行更具体的研究。
