Papas Maria G, Karatzas Pantelis S, Papanikolaou Ioannis S, Karamitopoulou Evanthia, Delicha Eumorphia M, Adler Andreas, Triantafyllou Konstantinos, Thomopoulou Georgia-Heleni, Patsouris Efstratios, Lazaris Andreas C
First Department of Pathology, Faculty of Medicine, University of Athens, Athens, Greece.
J Gastrointest Cancer. 2012 Sep;43(3):462-6. doi: 10.1007/s12029-011-9341-6.
LFA-1 is an adhesion molecule which belongs to the β2-integrin family. Overexpression of LFA-1 in hepatic natural killer cells has been associated with increased apoptosis of neoplastic cells in colorectal cancer (CRC); moreover, studies in CRC have linked LFA-1 overexpression in neoplastic cells with vascular intrusion through adhesion to endothelial cells, thus implying a possible role in creation of metastases.
We studied the expression of LFA-1 in a series of 82 patients with CRC. A standard three-step immunohistochemical analysis was performed on formalin-fixed, paraffin-embedded tissue sections. An IgG2a anti-CD11a monoclonal antibody was used. Cases were characterized according to clinicopathological variables including sex, age, tumor localization, size, grade, Dukes stage, wall invasion, and presence of metastatic lymph nodes (mLNs) or distal metastases.
LFA-1 was expressed at the primary tumor site in 51 cases and 6/33 cases with metastatic lymphnodes. In Dukes D cases (n = 4), only one case was LFA-1(+). LFA-1 expression at the primary tumor site was associated with the absence of metastatic disease and with Dukes B stage. However, in those cases with LFA-1 expression in cancer cells in mLNs, this was associated with its expression at the primary tumor site.
The positive association of LFA-1 expression in mLNs when the primary tumor site is also LFA-1(+) could imply an adaptation advantage of this specific cellular clone to its micro-environment, predisposing it to creation of mLNs, pointing to a role for LFA-1 in creation of mLNs in CRC.
淋巴细胞功能相关抗原-1（LFA-1）是一种属于β2整合素家族的黏附分子。肝自然杀伤细胞中LFA-1的过表达与结直肠癌（CRC）中肿瘤细胞凋亡增加有关；此外，CRC研究将肿瘤细胞中LFA-1的过表达与通过黏附内皮细胞导致的血管侵犯联系起来，从而暗示其在转移形成中可能发挥作用。
我们研究了82例CRC患者中LFA-1的表达情况。对福尔马林固定、石蜡包埋的组织切片进行标准的三步免疫组织化学分析。使用IgG2a抗CD11a单克隆抗体。根据临床病理变量对病例进行特征描述，包括性别、年龄、肿瘤定位、大小、分级、Dukes分期、肠壁侵犯以及有无转移性淋巴结（mLNs）或远处转移。
51例原发肿瘤部位及6/33例有转移性淋巴结的病例中LFA-1呈阳性表达。在Dukes D期病例（n = 4）中，仅1例LFA-1呈阳性。原发肿瘤部位LFA-1表达与无转移性疾病及Dukes B期相关。然而，在mLNs中癌细胞有LFA-1表达的病例中，这与原发肿瘤部位的表达相关。
当原发肿瘤部位LFA-1也呈阳性时，mLNs中LFA-1表达的正相关可能意味着这种特定细胞克隆对其微环境具有适应性优势，使其易于形成mLNs，表明LFA-1在CRC的mLNs形成中发挥作用。
