Junker Astrid, Junker Björn H
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany.
Methods Mol Biol. 2012;813:343-58. doi: 10.1007/978-1-61779-412-4_21.
Synthetic biology methods are routinely applied in the plant field as in other eukaryotic model systems. Several synthetic components have been developed in plants and an increasing number of studies report on the assembly into functional synthetic genetic circuits. This chapter gives an overview of the existing plant genetic networks and describes in detail the application of two systems for inducible gene expression. The ethanol-inducible system relies on the ethanol-responsive interaction of the AlcA transcriptional activator and the AlcR receptor resulting in the transcription of the gene of interest (GOI). In comparison, the translational fusion of GOI and the glucocorticoid receptor (GR) domain leads to the dexamethasone-dependent nuclear translocation of the GOI::GR protein. This chapter contains detailed protocols for the application of both systems in the model plants potato and Arabidopsis, respectively.
合成生物学方法在植物领域与其他真核模式系统中一样得到了常规应用。在植物中已经开发了几种合成元件，并且越来越多的研究报道了将其组装成功能性合成遗传回路。本章概述了现有的植物遗传网络，并详细描述了两种诱导型基因表达系统的应用。乙醇诱导系统依赖于AlcA转录激活因子与AlcR受体的乙醇应答相互作用，从而导致目的基因（GOI）的转录。相比之下，GOI与糖皮质激素受体（GR）结构域的翻译融合导致GOI::GR蛋白的地塞米松依赖性核转位。本章分别包含了这两种系统在模式植物马铃薯和拟南芥中应用的详细方案。
