Karlsson Maria, Weber Wilfried, Fussenegger Martin
Faculty of Biology, BIOSS Centre for Biological Signalling Studies, University of Freiburg, Freiburg, Germany.
Methods Mol Biol. 2012;813:359-76. doi: 10.1007/978-1-61779-412-4_22.
Advances in the development of molecular tools for the inducible control of transcription, translation, and protein degradation are the basis for the rapidly emerging design and construction of synthetic gene networks in mammalian cells.In this chapter, we describe such tools and how they can be integrated into a synthetic gene network with desired functionality. The network design and construction process is illustrated in the form of a detailed protocol for the implementation of a logic NOR gate based on an inducible promoter combined with an inducible protein degradation system.
用于转录、翻译和蛋白质降解的诱导控制的分子工具开发进展，是哺乳动物细胞中迅速兴起的合成基因网络设计与构建的基础。在本章中，我们描述了此类工具以及它们如何能够被整合到具有所需功能的合成基因网络中。网络设计与构建过程以基于诱导型启动子与诱导型蛋白质降解系统相结合来实现逻辑或非门的详细方案形式进行说明。
