Fujimoto S, Ishimitsu S, Kanazawa H, Ohara A, Hayakawa T
Laboratory of Biochemistry, Kyoto Pharmaceutical University, Japan.
Chem Pharm Bull (Tokyo). 1990 Jun;38(6):1653-5. doi: 10.1248/cpb.38.1653.
Sulfhydryl compounds significantly accelerated the hydroxylation of phenylalanine by stimulated polymorphonuclear leukocytes. The reduced form of glutathione (G-SH) was most effective. The hydroxylation reaction in the presence of G-SH was largely prevented by superoxide dismutase and hydroxyl radical scavengers. The results suggest that a much faster production of hydroxyl radical may occur in a reaction mixture containing both G-SH and stimulated polymorphonuclear leukocytes than in that containing stimulated polymorphonuclear leukocytes alone.
巯基化合物能显著加速受刺激的多形核白细胞对苯丙氨酸的羟化作用。还原型谷胱甘肽（G-SH）最为有效。超氧化物歧化酶和羟自由基清除剂能在很大程度上抑制G-SH存在时的羟化反应。结果表明，相较于仅含有受刺激多形核白细胞的反应混合物，同时含有G-SH和受刺激多形核白细胞的反应混合物中可能会更快地产生活性羟自由基。
