Tsuji T, Araki K, Naito K, Inoue J, Nacashima H
Acta Med Okayama. 1979 Feb;33(1):61-6.
Sera from 84 patients with chronic liver disease [CLD] (74 chronic active) and from 53 blood donors were tested immunochemically for anti-liver cell membrane antibody (LMAb). LMAb to rat liver tested by an indirect immunofluorescent technique was positive in 53.3% of CLD patients with positive HB surface antibody (HBsAb) and 40.0% of HBsAb positive blood donors. Pepsin digestion of the sera indicated that the binding between liver cell membrane and IgG was at the Fc site on the immunoglobulin. The sera with positive LMAb from HBsAb positive blood donors had elevated Clq-binding activity (Clq-BA). The LMAb to rat liver was a macro-molecular IgG (19-22S IgG) when assayed by Sephadex G-200 column chromatography and 5-40% sucrose density gradient ultracentrifugation. The results suggest that the LMAb in serum from a patient with chronic active liver disease may be an immune complex which consists of various antigens such as HB virus and its antibodies in serum.
对84例慢性肝病（CLD）患者（74例慢性活动性肝病）和53例献血者的血清进行免疫化学检测，以检测抗肝细胞膜抗体（LMAb）。采用间接免疫荧光技术检测，在乙肝表面抗体（HBsAb）阳性的CLD患者中，53.3%的患者抗大鼠肝LMAb呈阳性，在HBsAb阳性的献血者中，40.0%呈阳性。血清经胃蛋白酶消化表明，肝细胞膜与IgG之间的结合发生在免疫球蛋白的Fc位点。HBsAb阳性献血者中LMAb阳性的血清，其Clq结合活性（Clq-BA）升高。通过Sephadex G-200柱层析和5-40%蔗糖密度梯度超速离心法检测，抗大鼠肝LMAb为大分子IgG（19-22S IgG）。结果表明，慢性活动性肝病患者血清中的LMAb可能是一种免疫复合物，它由血清中的各种抗原如乙肝病毒及其抗体组成。
