Department of Environmental Agronomy and Crop Productions, University of Padua, Viale dell'Università 16, 35020 Legnaro (PD), Italy.
Biochem Biophys Res Commun. 2011 Dec 2;415(4):707-8. doi: 10.1016/j.bbrc.2011.10.148. Epub 2011 Nov 6.
An essential pre-requisite to perform sound quantitative real-time polymerase chain reaction (qPCR) assays is to design outstanding primer pairs. This means they must have a good efficiency and be not prone to produce multiple amplicons or primer dimer products. To circumvent these issues, several softwares are available to help primer design. Although satisfactory computer-aided primer design tools are available for standard PCR, less efforts were done to provide specific methods for selection of optimal primer pairs for qPCR. We have developed PRaTo a web-based tool that enables checking and ranking of primers pairs for their attitude to perform optimally and reliably when used in qPCR experiments. PRaTo is available at http://prato.daapv.unipd.it.
进行可靠的实时定量聚合酶链反应（qPCR）分析的一个基本前提是设计出色的引物对。这意味着它们必须具有良好的效率，并且不易产生多个扩增子或引物二聚体产物。为了解决这些问题，有几种软件可用于帮助设计引物。尽管有令人满意的用于标准 PCR 的计算机辅助引物设计工具，但在为 qPCR 选择最佳引物对方面却没有做太多工作。我们开发了一个名为 PRaTo 的基于网络的工具，该工具可用于检查和对引物对进行排序，以检查它们在 qPCR 实验中是否具有最佳和可靠的性能。PRaTo 可在 http://prato.daapv.unipd.it 上获得。
