Qiao J, Meng Q, Chen C, Xia X, Cai X, Ren Y, Zhang H
Department of Veterinary Medicine, Shihezi University, Shihezi, Xinjiang, PR China.
Acta Virol. 2011;55(4):303-10. doi: 10.4149/av_2011_04_303.
Canine distemper virus (CDV) is a highly contagious pathogen of dogs. Vaccination is an effective way to protect dogs from CDV infection, but occasionally fails. In the present study, a wild type (wt) CDV, named XJ2, was isolated from a dead vaccinated dog. The hemagglutinin (H) gene of the XJ2 was amplified and analyzed for the molecular characteristics including N-glycosylation sites, phylogenesis, hydrophobicity and epitopes. The data indicated that XJ2 was a genetic variant strain of CDV. CDV-sero-negative dogs were inoculated intranasally with XJ2, developed severe clinical symptoms and died, suggesting high virulence.
犬瘟热病毒（CDV）是犬类的一种高度传染性病原体。接种疫苗是保护犬类免受CDV感染的有效方法，但偶尔也会失效。在本研究中，从一只接种过疫苗但死亡的犬只中分离出一株野生型（wt）CDV，命名为XJ2。对XJ2的血凝素（H）基因进行了扩增，并分析了其分子特征，包括N-糖基化位点、系统发育、疏水性和表位。数据表明XJ2是CDV的一个基因变异株。用XJ2对CDV血清阴性犬进行鼻内接种，这些犬出现了严重的临床症状并死亡，表明其具有高毒力。
