Smith-Ravin J, Spencer J, Beverley P C, Isaacson P G
Imperial Cancer Research Fund-Human Tumour Immunology Group, Courtauld Institute, London, England, U.K.
Clin Exp Immunol. 1990 Oct;82(1):181-7. doi: 10.1111/j.1365-2249.1990.tb05424.x.
Two new monoclonal antibodies (MoAbs), UCL3D3 and UCL4D12 were obtained following immunization with follicular lymphoma (UCL3D3) or low-grade primary B cell gastric lymphoma cells (UCL4D12). In normal splenic white pulp, tonsil and small intestinal Peyer's patches, UCL4D12 recognizes marginal zone B cells and a subpopulation of follicle centre cells, whereas mantle zone B cells are UCL4D12 negative. In contrast, UCL3D3 recognizes mantle zone B cells and follicular dendritic cells, but not marginal zone B cells or follicle centre B cells. Double-immunofluorescence studies showed that in the splenic white pulp, these antibodies stain reciprocally. The majority of UCL3D3+ cells are sIgM+ and sIgD+ whereas a higher proportion of UCL4D12+ cells express surface IgM (sIgM) but not surface IgD (sIgD). Less than 10% of splenic B cells express both 3D3 and 4D12 antigens. None of the cell lines tested expressed either antigen. Functional studies showed that both antigens play a role in B cell activation as the MoAbs increase the mitogenic effect of Staphylococcus aureus Cowan I on tonsil B cells. This effect was maximal at 72 h in culture. TPA activation was reduced, and no effect was observed with anti-immunoglobulin (anti mu) or CDw40 (G28.5). UCL3D3 and UCL4D12 did not show any stimulatory effect on their own. Biochemical studies show that both MoAbs recognize proteins of 80-90 kD under reducing conditions. These two MoAbs appear to recognize new B cell surface antigens which may be useful for identifying subpopulations of B cells.
用滤泡性淋巴瘤（UCL3D3）或低级别原发性B细胞胃淋巴瘤细胞（UCL4D12）免疫后，获得了两种新的单克隆抗体（MoAbs），即UCL3D3和UCL4D12。在正常脾白髓、扁桃体和小肠派伊尔结中，UCL4D12识别边缘区B细胞和滤泡中心细胞的一个亚群，而套区B细胞为UCL4D12阴性。相比之下，UCL3D3识别套区B细胞和滤泡树突状细胞，但不识别边缘区B细胞或滤泡中心B细胞。双重免疫荧光研究表明，在脾白髓中，这些抗体呈相互染色。大多数UCL3D3+细胞为sIgM+和sIgD+，而较高比例的UCL4D12+细胞表达表面IgM（sIgM）但不表达表面IgD（sIgD）。脾B细胞中不到10%的细胞同时表达3D3和4D12抗原。所测试的细胞系均未表达这两种抗原。功能研究表明，这两种抗原在B细胞活化中均起作用，因为单克隆抗体增强了金黄色葡萄球菌Cowan I对扁桃体B细胞的促有丝分裂作用。这种作用在培养72小时时最大。TPA活化降低，抗免疫球蛋白（抗μ）或CDw40（G28.5）未观察到作用。UCL3D3和UCL4D12自身未显示任何刺激作用。生化研究表明，在还原条件下，这两种单克隆抗体均识别80 - 90 kD的蛋白质。这两种单克隆抗体似乎识别新的B细胞表面抗原，这可能有助于识别B细胞亚群。
