Kuritani T, Cooper M D
J Exp Med. 1982 May 1;155(5):1561-6. doi: 10.1084/jem.155.5.1561.
Surface immunoglobulin D (IgD)-positive lymphocytes precoated with monoclonal anti-delta antibody were selectively removed from blood mononuclear cell preparations by "panning" and by fluorescence-activated cell sorter. The depletion of sIgD+ cells did not significantly affect plasma cell responses to pokeweed mitogen PWM). PWM-responsive B cells lacking sIgD and mouse erythrocyte receptors preferentially sedimented in lower density fractions of a discontinuous Percoll gradient, and sIgD-negative B cells were found to have a larger mean diameter than IgD-positive cells. We conclude that PWM-responsive B cells represent a distinct subpopulation of relatively large cells that have ceased to express receptors for mouse erythrocytes and surface IgD.
通过“淘选”和荧光激活细胞分选仪，从血液单核细胞制剂中选择性去除预先用单克隆抗δ抗体包被的表面免疫球蛋白D（IgD）阳性淋巴细胞。sIgD +细胞的去除对浆细胞对商陆有丝分裂原（PWM）的反应没有显著影响。缺乏sIgD和小鼠红细胞受体的PWM反应性B细胞优先沉淀在不连续Percoll梯度的较低密度级分中，并且发现sIgD阴性B细胞的平均直径大于IgD阳性细胞。我们得出结论，PWM反应性B细胞代表了一个相对较大细胞的独特亚群，这些细胞已不再表达小鼠红细胞和表面IgD的受体。
