Xu Yinghua, Bonar Fiona, Murrell George A C
Orthopaedic Research Institute, St George Hospital Campus, University of New South Wales, Sydney, NSW, Australia.
Sports Med Arthrosc Rev. 2011 Dec;19(4):354-9. doi: 10.1097/JSA.0b013e318229d7e3.
The aim of this study was to determine whether there are more nerves in tendinopathic human tendon, and if so, where are they located. Tendon biopsies were collected from normal, tendinopathic, and torn human rotator cuff tendons and then analyzed using immunohistochemistry and antibodies against a general nerve marker (protein gene product 9.5, PGP9.5), a nerve regeneration marker (growth-associated protein 43, GAP43), and an endothelial cell marker (CD34). Nerve fibers exhibiting PGP9.5 or GAP43 immunoreactivity were often observed intimately in association with tiny blood vessels in the endotendineum of tendinopathic tendons. The expression of PGP9.5 and GAP43 were significantly higher in tendinopathic tendon compared with control tendon and torn tendon. These data support the hypothesis that early tendinopathy is associated with increases of newly grown nerve fibers and blood vessels inside and around tendinopathic tendon, and these may be the source of pain in tendinopathy.
本研究的目的是确定患有肌腱病的人体肌腱中是否存在更多神经，若存在，它们位于何处。从正常、患有肌腱病以及撕裂的人体肩袖肌腱中采集肌腱活检样本，然后使用免疫组织化学方法以及针对一般神经标志物（蛋白基因产物9.5，PGP9.5）、神经再生标志物（生长相关蛋白43，GAP43）和内皮细胞标志物（CD34）的抗体进行分析。在患有肌腱病的肌腱的内腱膜中，经常观察到显示PGP9.5或GAP43免疫反应性的神经纤维与微小血管紧密相连。与对照肌腱和撕裂肌腱相比，PGP9.5和GAP43在患有肌腱病的肌腱中的表达显著更高。这些数据支持了以下假设：早期肌腱病与患有肌腱病的肌腱内部及周围新生长的神经纤维和血管增加有关，而这些可能是肌腱病疼痛的来源。
