Health Protection Agency, Porton Down, Salisbury, Wiltshire SP4 0JG, UK.
J Gen Virol. 2012 Mar;93(Pt 3):560-564. doi: 10.1099/vir.0.038455-0. Epub 2011 Nov 16.
Hazara virus (HAZV) is closely related to the Crimean-Congo hemorrhagic fever virus (CCHFV). HAZV has not been reported to cause human disease; work with infectious material can be carried out at containment level (CL)-2. By contrast, CCHFV causes a haemorrhagic fever in humans and requires CL-4 facilities. A disease model of HAZV infection in mice deficient in the type I interferon receptor is reported in this study. Dose-response effects were seen with higher doses, resulting in a shorter time to death and earlier detection of viral loads in organs. The lowest dose of 10 p.f.u. was still lethal in over 50 % of the mice. Histopathological findings were identified in the liver, spleen and lymph nodes, with changes similar to a recent mouse model of CCHFV infection. The findings demonstrate that inoculation of mice with HAZV may act as a useful surrogate model for the testing of antiviral agents against CCHFV.
哈扎拉病毒（HAZV）与克里米亚-刚果出血热病毒（CCHFV）密切相关。HAZV 尚未被报道引起人类疾病；可以在生物安全防护等级 2（CL-2）下进行与传染性材料相关的工作。相比之下，CCHFV 会引起人类出血热，需要在生物安全防护等级 4（CL-4）设施下进行操作。本研究报道了一种在缺乏 I 型干扰素受体的小鼠中感染 HAZV 的疾病模型。高剂量下观察到剂量反应效应，导致死亡时间缩短，器官中病毒载量更早被检测到。10 个组织培养感染剂量（p.f.u.）的最低剂量仍导致超过 50%的小鼠死亡。在肝脏、脾脏和淋巴结中发现了组织病理学变化，与最近的 CCHFV 感染小鼠模型的变化相似。这些发现表明，用 HAZV 接种小鼠可能是一种有用的替代模型，可用于测试针对 CCHFV 的抗病毒药物。
