Department of Medicine, Institute of Clinical Medicine, Helsinki University Central Hospital, Haartmaninkatu 8, FI-00029 HUS, Finland.
J Biomater Appl. 2013 Mar;27(7):862-71. doi: 10.1177/0885328211428094. Epub 2011 Nov 15.
Osteogenic responses of human mesenchymal stromal cells (hMSCs) were compared on square-patterned, inverse square-patterned, and planar titanium, chromium, diamond-like carbon (DLC), and tantalum; hypothesis was that both the materials and patterns affect osteogenesis. Samples were produced using photolithography and physical vapor deposition. Early-marker alkaline phosphatase (ALP) and mid-markers, small body size and mothers against decapentaplegic-related protein-1 (SMAD1), runt-related transcription factor-2 (RUNX2), and osteopontin were studied using quantitative real-time polymerase chain reaction. ALP and hydroxyapatite, were colorimetrically studied. ALP reached highest values on both patterned titanium samples, but mid-markers disclosed that it was already lagging behind planar and inverse patterned tantalum. Hydroxyapatite formation disclosed that osteo-induced hMSCs passed all the differentiation stages (except on planar chromium). Presence of hydroxyapatite disclosed that both types of patterning promoted (p < 0.001) osteogenesis compared to planar samples. Results suggest that the osseocompatibility/integration of implants could be improved by changing the monotonous and featureless implant-host interface into micro-patterned interface to provide physical differentiation cues.
比较了人骨髓间充质基质细胞 (hMSCs) 在方型图案、倒方型图案、平面钛、铬、类金刚石碳 (DLC) 和钽上的成骨反应；假设材料和图案都会影响成骨。使用光刻和物理气相沉积生产样品。使用定量实时聚合酶链反应研究早期标志物碱性磷酸酶 (ALP) 和中期标志物、小体型和抗凋亡蛋白聚糖相关蛋白-1 (SMAD1)、 runt 相关转录因子-2 (RUNX2) 和骨桥蛋白。通过比色法研究 ALP 和羟磷灰石。ALP 在两种图案化钛样品上均达到最高值，但中期标志物表明其已落后于平面和倒图案化钽。羟磷灰石的形成表明，诱导成骨的 hMSCs 已经通过了所有分化阶段（除了平面铬）。存在羟磷灰石表明与平面样品相比，两种类型的图案化都促进了（p<0.001）成骨。结果表明，通过将单调无特征的植入物-宿主界面改变为微图案化界面，可以为物理分化提供线索，从而提高植入物的骨相容性/整合性。
