Department of Anatomy, Institute of Biomedicine, University of Helsinki, Helsinki, Finland.
J Biomed Mater Res A. 2010 Mar 15;92(4):1606-13. doi: 10.1002/jbm.a.32486.
Staphylococcus aureus device-related infection is a common complication in implantology. Bacterial adhesion on implant surfaces is the initial step in the infective process. The aim was to develop a method suitable for quantitative bacterial adherence studies and to test a new diamond-like carbon (DLC) coating against commonly used metallic biomaterials with regards to Staphylococcus aureus adhesion. Patterned silicon chips with spots of tantalum, titanium, chromium, and DLC were produced using ultraviolet lithography and physical vapor deposition. These patterned chips were used as such or glued to array plates, pretreated with serum and exposed to S. aureus (S-15981) for 90 min, followed by acridine orange staining and fluorescence microscopy. An adhesion index showed that the ranking order of the biomaterials was titanium, tantalum, chromium, and DLC, with the DLC being clearly most resistant against colonization with S. aureus. Micropatterned surfaces are useful for quantitative comparison of bacterial adherence on different biomaterials. In the presence of serum, DLC is superior in its ability to resist adhesion and colonization by S. aureus compared with the commonly used biomaterial metals tantalum, titanium, and chromium.
金黄色葡萄球菌器械相关感染是植入物领域的常见并发症。细菌在植入物表面的黏附是感染过程的初始步骤。本研究旨在开发一种适用于定量细菌黏附研究的方法，并针对金黄色葡萄球菌黏附，测试一种新的类金刚石碳（DLC）涂层对常用金属生物材料的效果。采用紫外光刻和物理气相沉积技术，在硅片上制作了钽、钛、铬和 DLC 点的图案化芯片。这些图案化芯片可以直接使用，也可以粘贴到阵列板上，先用血清预处理，然后将其暴露于金黄色葡萄球菌（S-15981）中 90 分钟，再用吖啶橙染色，荧光显微镜观察。黏附指数显示，生物材料的排序为钛、钽、铬和 DLC，DLC 对金黄色葡萄球菌的定植具有明显的抵抗力。微图案化表面可用于不同生物材料细菌黏附的定量比较。在血清存在的情况下，与常用的生物材料金属钽、钛和铬相比，DLC 具有更强的抗金黄色葡萄球菌黏附和定植能力。
