[双嘧达莫和抗坏血酸对细胞培养中人类干扰素抗病毒活性的影响]

[Effect of dibasol and ascorbic acid on the antiviral activity of human interferon in cell culture].

作者信息

Povolotskiĭ Ia L, Krivokhatskaia L D

出版信息

Antibiotiki. 1979 Apr;24(4):291-4.

PMID:220907

Abstract

The antiviral effect of human lymphocytic interferon was studied in the primary culture of human embryonic fibroblasts in the presence of dibazol and ascorbic acid. It was found that dibazol and ascorbic acid in concentrations of 5 and 10 gamma/ml respectively were capable of increasing the antiviral effect of human interferon in homological cells. The assays of 13 lots of interferon showed that its average titer in the experiments with ascorbic acid was 2.5 times higher than that in the control. The assays of 21 lots of interferon showed that its average titer in the experiments with dibazol was 3 times higher than that in the control. It is suggested that an increase in the protective properties of interferon in the presence of dibazol and ascorbic acid is connected with their capacity for stimulating the intracellular production of DNA and protein. The data obtained indicate that dibazol and ascorbic acid may be recommended in the complex of therapy and prophylaxis of antiviral infections.

摘要

在存在地巴唑和抗坏血酸的情况下，在人胚胎成纤维细胞原代培养物中研究了人淋巴细胞干扰素的抗病毒作用。发现地巴唑和抗坏血酸的浓度分别为5和10微克/毫升时，能够增强人干扰素在同源细胞中的抗病毒作用。对13批干扰素的检测表明，在使用抗坏血酸的实验中其平均效价比对照组高2.5倍。对21批干扰素的检测表明，在使用地巴唑的实验中其平均效价比对照组高3倍。有人认为，在地巴唑和抗坏血酸存在的情况下干扰素保护特性的增强与其刺激细胞内DNA和蛋白质产生的能力有关。所获得的数据表明，地巴唑和抗坏血酸可推荐用于抗病毒感染的治疗和预防综合措施中。