Effect of amino acids on the expression of antiviral activity of different types of human interferon. II. Effects of various amino acid pairs.

Seven amino acids which influence the antiviral activity of IFN-alpha were tested in various pairs on human leukocyte and immune IFN and on their synergistic antiviral effect. Different kinds of interactions were observed between amino acids, ranging from extinction through competition to synergism. In some cases, different types of interactions could be observed in relation to HuIFN-alpha and HuIFN-gamma. It has been reported that certain amino acids influence the antiviral activity of human interferons (IFNs). Some of them are even capable of affecting synergistic cooperation between HuIFN-alpha and HuIFN-gamma. In our experiments we tested whether these effects can be modified further by applying two amino acids simultaneously. We hoped to shed light on the problem of their mode of action by analysing their interactions.