Department of Odontology, Umeå University, S-901 87 Umeå, Sweden.
Clin Oral Implants Res. 2013 Mar;24(3):329-35. doi: 10.1111/j.1600-0501.2011.02334.x. Epub 2011 Nov 1.
To systematically investigate the biological interface of Bio-Oss by analysing dissolution-precipitation behaviour and osteogenic responses using in vitro experimental systems.
Different concentrations (1-100 mg/ml) of Bio-Oss were incubated in cell culture medium for 24 h before elemental concentrations for calcium, phosphorus and silicon in the medium were analysed with inductive coupled plasma-optical emission spectroscopy. Radioactive calcium-45 isotope labelling technique was used to study possible precipitation of calcium on the Bio-Oss particle. Biological interface of Bio-Oss was studied in osteogenic experiments using mineralization medium and three different sources of cells (primary mouse bone marrow stromal cells, primary rat calvarial cells and MC3T3-E1 mouse pre-osteoblast cell line). Cells were fixed and stained with Toulidine blue, von Kossa or Alizarin Red staining for confirmation of extracellular matrix mineralization.
Elemental analysis of the cell culture medium demonstrated a significant decrease of calcium and phosphorus and a dose-dependent release of silicon to the medium after incubation with Bio-Oss. A significant decrease of calcium and phosphorus in the medium occurred even at low concentrations of Bio-Oss. Uptake of calcium on the Bio-Oss particle was confirmed with radioactive calcium-45 isotope labelling technique. In osteogenic experiments with Bio-Oss (<1 mg/ml), matrix mineralization around the Bio-Oss particles were demonstrated in all three cell types with von Kossa and Alizarin Red staining.
Dissolution-precipitation reactions occur at the surface of Bio-Oss, and osteogenic responses are seen at the biological interface. The concentration of Bio-Oss is a key factor for the experimental in vitro results, and may also have implications for the clinic.
通过分析体外实验系统中的溶解-沉淀行为和成骨反应，系统研究 Bio-Oss 的生物界面。
将不同浓度（1-100mg/ml）的 Bio-Oss 在细胞培养液中孵育 24 小时，然后用电感耦合等离子体-光学发射光谱法分析培养液中钙、磷和硅的元素浓度。使用放射性钙-45 同位素标记技术研究钙在 Bio-Oss 颗粒上可能的沉淀。使用矿化培养基和三种不同来源的细胞（原代小鼠骨髓基质细胞、原代大鼠颅骨细胞和 MC3T3-E1 小鼠前成骨细胞系）进行成骨实验，研究 Bio-Oss 的生物界面。用甲苯胺蓝、von Kossa 或茜素红染色固定和染色，以确认细胞外基质矿化。
细胞培养液的元素分析表明，孵育后培养液中钙和磷的浓度显著降低，硅呈剂量依赖性释放到培养液中。即使在低浓度的 Bio-Oss 下，培养液中钙和磷的浓度也显著降低。放射性钙-45 同位素标记技术证实了 Bio-Oss 颗粒上钙的摄取。在 Bio-Oss（<1mg/ml）的成骨实验中，von Kossa 和茜素红染色显示，所有三种细胞类型在 Bio-Oss 颗粒周围均有基质矿化。
在 Bio-Oss 表面发生溶解-沉淀反应，在生物界面观察到成骨反应。Bio-Oss 的浓度是实验体外结果的关键因素，也可能对临床有影响。
