Clinic of Preventive Dentistry, Periodontology and Cariology, Center of Dental Medicine, University of Zürich, Zürich, Switzerland.
Clin Oral Implants Res. 2013 Feb;24(2):149-57. doi: 10.1111/j.1600-0501.2011.02315.x. Epub 2011 Nov 1.
Moldable in situ self-stabilizing and hardening bone graft materials facilitate handling and may be suitable for membrane-free bone regeneration methods. This study aimed to compare two moldable synthetic calcium phosphate materials in a rabbit calvarial defect model.
In 12 New Zealand white rabbits, four evenly distributed 6 mm diameter defects were drilled in the calvarial bone. Three filler materials were randomly applied to 48 defects: an in situ hardening polylactide-coated ß-tricalcium phosphate (TCP), an in situ hardening polylactide-coated biphasic calcium phosphate (BCP), and a granular deproteinized bovine bone matrix (DBBM, positive control). One defect remained untreated and served as a negative control. Six animals were sacrificed after 4 weeks, and the remaining animals were sacrificed after 16 weeks. Biocompatibility, bone graft substitute integration and resorption, bone formation, defect bridging, and height of reconstructed hard tissue were assessed histologically and histomorphometrically.
All tested materials showed good biocompatibility. Semi-quantitative analysis and pair-wise comparison suggested that BCP was more efficient in centripetal bone formation when compared with TCP. After 4 weeks, significantly more bone had formed in the defects treated with either TCP or BCP materials compared with the untreated sites. BCP and DBBM did not show macroscopic signs of degradation, whereas the TCP material was partially resorbed after 16 weeks. Otherwise, no major differences were detected between the three materials.
The moldable, synthetic calcium phosphates are safe and suitable bone graft substitutes with outcomes that are comparable to the control material.
可塑形的原位自稳定和硬化骨移植材料便于操作，可能适用于无膜骨再生方法。本研究旨在比较两种可塑形的合成磷酸钙材料在兔颅骨缺损模型中的应用。
在 12 只新西兰白兔中，颅骨骨头上均匀分布钻了 4 个 6 毫米直径的缺损。将 3 种填充材料随机应用于 48 个缺损：原位硬化聚乳酸涂层β-磷酸三钙（TCP）、原位硬化聚乳酸涂层双相磷酸钙（BCP）和颗粒脱蛋白牛骨基质（DBBM，阳性对照）。一个缺损未处理，作为阴性对照。6 只动物在 4 周后处死，其余动物在 16 周后处死。通过组织学和组织形态计量学评估生物相容性、骨移植替代物的整合和吸收、骨形成、缺损桥接以及重建硬组织的高度。
所有测试材料均显示出良好的生物相容性。半定量分析和两两比较表明，与 TCP 相比，BCP 在向心性骨形成方面更有效。4 周后，与未处理部位相比，用 TCP 或 BCP 材料处理的缺陷中形成的骨量明显更多。BCP 和 DBBM 没有表现出明显的降解迹象，而 TCP 材料在 16 周后部分被吸收。否则，三种材料之间没有发现明显差异。
可塑形的合成磷酸钙是安全且适合的骨移植替代物，其结果与对照材料相当。
