Finger Stadler Amanda, Musskopf Marta Liliana, Gohel Vishal, Reside Jonathan, Everett Eric, Miguez Patricia, Susin Cristiano
Department of Periodontology, Endodontics and Dental Hygiene, Adams School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Department of Biomedical Sciences, Adams School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27517, USA.
J Funct Biomater. 2025 Jun 6;16(6):214. doi: 10.3390/jfb16060214.
to evaluate the bone regeneration capacity of two alloplastic biomaterials in a critical-size rat calvarial defect model.
A total of 80 rats were randomized into 8 groups of 10 animals each. An Ø8 mm, critical-size calvarial defect was created, and the following treatments were randomly allocated: sham surgery, deproteinized bovine bone mineral (DBBM) + collagen membrane (CM), poly-(lactic-co-glycolic-acid) (PLGA)-coated pure phase β-tricalcium phosphate (β-TCP), or PLGA-coated 60% hydroxyapatite (HA):40%β-TCP. Animals were allowed to heal for 2 and 6 weeks. Microcomputed tomography (μCT) was used to evaluate mineralized tissue and biomaterial displacement. Histological samples were used to evaluate new bone formation.
μCT analysis showed no significant differences among groups for total volume of mineralized tissue or residual biomaterials. DBBM + CM showed significantly increased horizontal biomaterial displacement at 2 weeks but not at 6 weeks. Histological analysis showed that sham surgery had a significantly higher percentage of bone area fraction than the DBBM + CM and PLGA + β-TCP at 2 weeks, but not at 6 weeks. Residual biomaterial area fraction showed no significant differences among experimental groups at any healing time.
The alloplastic biomaterials showed suitable construct integrity and retention in the defect. All biomaterials were associated with limited new bone formation comparable to the sham surgery control.
在大鼠颅骨临界尺寸缺损模型中评估两种异体生物材料的骨再生能力。
总共80只大鼠被随机分为8组，每组10只动物。制造一个直径8毫米的颅骨临界尺寸缺损，并随机分配以下治疗方法：假手术、脱蛋白牛骨矿物质（DBBM）+胶原膜（CM）、聚（乳酸-乙醇酸）（PLGA）涂层的纯相β-磷酸三钙（β-TCP）或PLGA涂层的60%羟基磷灰石（HA）：40%β-TCP。让动物愈合2周和6周。使用微型计算机断层扫描（μCT）评估矿化组织和生物材料移位情况。组织学样本用于评估新骨形成情况。
μCT分析显示，矿化组织总体积或残余生物材料在各组之间无显著差异。DBBM+CM在2周时水平生物材料移位显著增加，但在6周时未增加。组织学分析显示，假手术在2周时骨面积分数百分比显著高于DBBM+CM和PLGA+β-TCP，但在6周时并非如此。在任何愈合时间，残余生物材料面积分数在实验组之间均无显著差异。
异体生物材料在缺损处显示出合适的结构完整性和留存率。所有生物材料与假手术对照组相比，新骨形成均有限。
