Pasley J N, Rayford P L
Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock 72205.
Dig Dis Sci. 1990 Oct;35(10):1265-70. doi: 10.1007/BF01536417.
Altered protein diets and circadian rhythms of gastrin and cholecystokinin (CCK) were investigated in 126 male and 126 female Sprague-Dawley rats acclimated for two weeks to a 12:12 hr light-dark cycle. Rats were divided equally and fed low-protein (8%), high-protein (64%) or normal protein (27%) diets for four weeks. All animals were fasted for 24 hr prior to blood collections. Blood samples were collected at 4-hr intervals for 24 hr for determination of plasma gastrin and CCK using specific radioimmunoassays. A significant rhythm for gastrin was detected in males on normal and low-protein diets (P less than 0.03) and in females on low-protein diets (P less than .02). A significant rhythm for CCK was detected (P less than 0.05) in rats of both sexes fed normal and high-protein diets. Mean plasma levels of both peptides were lower in females than males. In a separate study, food intake and body weight were monitored in male rats receiving the three diets over 21 days. Animals on the low-protein diet exhibited reduced food intake and body weight compared to rats fed the normal or high-protein diets.
在126只雄性和126只雌性斯普拉格-道利大鼠中研究了改变的蛋白质饮食以及胃泌素和胆囊收缩素（CCK）的昼夜节律，这些大鼠已适应12:12小时的明暗循环两周。将大鼠平均分成三组，分别喂食低蛋白（8%）、高蛋白（64%）或正常蛋白（27%）饮食四周。在采集血液样本前，所有动物均禁食24小时。每隔4小时采集一次血样，共采集24小时，使用特定的放射免疫分析法测定血浆胃泌素和CCK。在食用正常蛋白和低蛋白饮食的雄性大鼠（P<0.03）以及食用低蛋白饮食的雌性大鼠（P<0.02）中检测到胃泌素的显著节律。在食用正常蛋白和高蛋白饮食的雌雄大鼠中均检测到CCK的显著节律（P<0.05）。两种肽的平均血浆水平在雌性中均低于雄性。在另一项研究中，对接受三种饮食的雄性大鼠进行了21天的食物摄入量和体重监测。与喂食正常蛋白或高蛋白饮食的大鼠相比，食用低蛋白饮食的动物食物摄入量和体重均降低。
