Green G M, Levan V H, Liddle R A
Am J Physiol. 1986 Jul;251(1 Pt 1):G70-4. doi: 10.1152/ajpgi.1986.251.1.G70.
The relationship among plasma cholecystokinin (CCK), pancreatic growth, and food intake was studied in rats over a 2-wk period of adaptation from a very low-protein to a very high-protein diet. Rats adapted to a control diet (5% casein) were killed at 0900 (without fasting) at 0 h, 12 h, 24 h, 48 h, 7 days, or 14 days after transfer to a high-protein diet (75% casein). CCK was measured by bioassay using isolated pancreatic acini. Plasma CCK in high protein-fed rats was increased approximately threefold in the first 24 h, but returned to control (approximately 2.5 pM) values by day 7. Pancreatic weight, DNA, protein, and chymotrypsin(ogen) significantly increased to maximal values by day 7 in high protein-fed rats. Food intake in high protein-fed rats was inhibited by 47% after 24 h but returned to control values by day 7. The results indicate that high-protein diets initially increase CCK release and increase pancreatic protease secretory capacity and that, when pancreatic protease secretion is sufficient to match protein digestive requirements, the stimulus for CCK secretion is reduced and plasma CCK returns to normal. The pronounced but transient inhibition of food intake in high protein-fed rats is consistent with a role for CCK in regulation of food intake.
在大鼠从极低蛋白饮食适应到极高蛋白饮食的2周期间，研究了血浆胆囊收缩素（CCK）、胰腺生长和食物摄入量之间的关系。适应对照饮食（5%酪蛋白）的大鼠在转移到高蛋白饮食（75%酪蛋白）后的0小时、12小时、24小时、48小时、7天或14天的09:00（未禁食）处死。使用分离的胰腺腺泡通过生物测定法测量CCK。高蛋白喂养大鼠的血浆CCK在最初24小时内增加约三倍，但到第7天恢复到对照值（约2.5 pM）。高蛋白喂养大鼠的胰腺重量、DNA、蛋白质和胰凝乳蛋白酶（原）在第7天显著增加至最大值。高蛋白喂养大鼠的食物摄入量在24小时后受到47%的抑制，但到第7天恢复到对照值。结果表明，高蛋白饮食最初会增加CCK释放并增加胰腺蛋白酶分泌能力，并且当胰腺蛋白酶分泌足以满足蛋白质消化需求时，CCK分泌的刺激会减少，血浆CCK恢复正常。高蛋白喂养大鼠食物摄入量的显著但短暂的抑制与CCK在食物摄入调节中的作用一致。
