Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Oral Dis. 2012 Mar;18(2):206-12. doi: 10.1111/j.1601-0825.2011.01871.x. Epub 2011 Nov 18.
The effect of growth differentiation factor 5 and bone morphogenetic protein 2 on human periodontal ligament-derived cells was investigated with special reference to tendo/ligamentogenesis-related markers.
Effects of each factor were analyzed by quantitative PCR for scleraxis and tenomodulin and by western blotting for scleraxis. After exposure to those factors, STRO-1-positive and STRO-1-negative fractions of human periodontal ligament tissues were isolated with an immunomagnetic cell sorting system, and the expression of scleraxis in each fraction was analyzed by western blotting. Non-separated crude cells were used as a control.
Growth differentiation factor 5 and bone morphogenetic protein 2 did not increase alkaline phosphatase activity in crude periodontal ligament-derived cells. Growth differentiation factor 5, but not bone morphogenetic protein 2, increased the expression of scleraxis in crude, STRO-1-positive and STRO-1-negative periodontal ligament-derived cells. The expression of scleraxis in STRO-1-positive periodontal ligament-derived cells was significantly less compared to that in crude P2 and STRO-1-negative periodontal ligament-derived cells.
Growth differentiation factor 5 induced the expression of scleraxis and may enhance tendo/ligamentogenesis in human periodontal ligament-derived cells. The expression of scleraxis was higher in STRO-1-negative fraction, suggesting more differentiated state of the cells.
研究生长分化因子 5 和骨形态发生蛋白 2 对人牙周膜源性细胞的影响，特别关注与腱/韧带发生相关的标记物。
通过定量 PCR 分析每个因子对硬骨形成蛋白和肌腱蛋白聚糖的影响，并通过 Western blot 分析硬骨形成蛋白。在暴露于这些因子后，使用免疫磁细胞分选系统分离人牙周膜组织中 STRO-1 阳性和 STRO-1 阴性细胞，并通过 Western blot 分析每个部分的硬骨形成蛋白表达。未分离的粗细胞作为对照。
生长分化因子 5 和骨形态发生蛋白 2 不会增加粗牙周膜源性细胞中的碱性磷酸酶活性。生长分化因子 5 但不是骨形态发生蛋白 2 增加了粗、STRO-1 阳性和 STRO-1 阴性牙周膜源性细胞中硬骨形成蛋白的表达。与粗 P2 和 STRO-1 阴性牙周膜源性细胞相比，STRO-1 阳性牙周膜源性细胞中硬骨形成蛋白的表达明显减少。
生长分化因子 5 诱导硬骨形成蛋白的表达，并可能增强人牙周膜源性细胞中的腱/韧带发生。STRO-1 阴性部分中硬骨形成蛋白的表达较高，表明细胞的分化状态更高。
