Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, Osaka, Japan.
J Appl Microbiol. 2012 Feb;112(2):404-11. doi: 10.1111/j.1365-2672.2011.05194.x. Epub 2011 Dec 8.
To investigate the effects of the combined application of an N-acyl homoserine lactone (HSL) analog and antibiotics on biofilms of Porphyromonas gingivalis, a major pathogen of periodontal disease.
Antibiotics used were cefuroxime, ofloxacin and minocycline. A flow-cell model was used for biofilm formation. Samples were divided into four groups: control, analog-treated, antibiotic-treated and combined application groups. Biofilm cell survival was determined using adenosine triphosphate (ATP) bioluminescence and confocal laser microscopy (CLSM). In the combined application group, the ATP count in biofilm cells was significantly decreased compared with the antibiotic-treated group (Games-Howell test, P < 0·05). A combination of cefuroxime and the analog was most effective against the P. gingivalis biofilm. CLSM observations revealed that the proportion of dead cells was highest in the combined application group.
The combined application of the N-acyl HSL analog and antibiotics was effective at reducing the viability of P. gingivalis cells in biofilms.
The combined application of the N-acyl HSL analog and antibiotics may be successful for eradicating infections involving bacterial biofilms, such as periodontitis.
研究 N-酰基高丝氨酸内酯（HSL）类似物与抗生素联合应用对牙周病主要致病菌牙龈卟啉单胞菌生物膜的影响。
所用抗生素为头孢呋辛、氧氟沙星和米诺环素。采用流室模型进行生物膜形成。将样品分为四组：对照组、类似物处理组、抗生素处理组和联合应用组。使用三磷酸腺苷（ATP）生物发光和共聚焦激光显微镜（CLSM）测定生物膜细胞的存活情况。在联合应用组中，与抗生素处理组相比，生物膜细胞中的 ATP 计数明显减少（Games-Howell 检验，P<0.05）。头孢呋辛和类似物的联合应用对牙龈卟啉单胞菌生物膜最有效。CLSM 观察显示，联合应用组中死亡细胞的比例最高。
N-酰基 HSL 类似物与抗生素联合应用可有效降低生物膜中牙龈卟啉单胞菌细胞的活力。
N-酰基 HSL 类似物与抗生素的联合应用可能成功根除涉及细菌生物膜的感染，如牙周炎。
