Department of Biophysics, School of Medicine, Akdeniz University, Antalya, Turkey.
Dermatol Surg. 2012 Feb;38(2):215-23. doi: 10.1111/j.1524-4725.2011.02174.x. Epub 2011 Oct 14.
Elastic light single-scattering spectroscopy (ELSSS) is a noninvasive and real-time technique that has been used to differentiate tumors from surrounding nontumor tissue in animal models and humans.
To investigate potential application of ELSSS as an adjunctive tool for noninvasive, in vivo, real-time differentiation of malignant and benign skin lesions and to detect positive surgical margins of excised biopsy samples.
In vivo spectroscopic measurements were performed on 28 lesions in 23 patients. The distribution of the lesions was as follows: nine basal cell carcinoma (BCC), four melanoma, two squamous cell carcinoma (SCC), and 13 benign lesions. Intraoperative margin assessments were performed on the 28 biopsy samples using ELSSS.
The sign of the spectral slopes was positive for benign and negative for malignant tissues. It was used as a discrimination parameter between malignant and benign lesions with a sensitivity and specificity of 87% and 85%, respectively. Sensitivity and specificity of the system in detecting positive surgical margins on 14 excised biopsy samples were 80% and 90%, respectively.
ELSSS has the potential for use as an adjunctive tool to reduce the number of negative biopsies and to detect positive surgical margins in real time.
弹性光单次散射光谱学（ELSSS）是一种非侵入性和实时技术，已被用于在动物模型和人体中区分肿瘤与周围非肿瘤组织。
研究 ELSSS 作为一种辅助工具，用于非侵入性、体内、实时区分恶性和良性皮肤病变，并检测切除活检样本的阳性手术切缘的潜在应用。
对 23 名患者的 28 个病变进行了体内光谱测量。病变的分布如下：基底细胞癌（BCC）9 个，黑色素瘤 4 个，鳞状细胞癌（SCC）2 个，良性病变 13 个。使用 ELSSS 对 28 个活检样本进行了术中切缘评估。
良性病变的光谱斜率符号为正，恶性组织的光谱斜率符号为负。该斜率符号可作为区分良恶性病变的判别参数，其灵敏度和特异性分别为 87%和 85%。该系统在检测 14 个切除活检样本中的阳性手术切缘的灵敏度和特异性分别为 80%和 90%。
ELSSS 有可能作为一种辅助工具，减少阴性活检的数量，并实时检测阳性手术切缘。
