Department of Biophysics, School Of Medicine, Alaaddin Keykubat University, Alanya, Antalya, Turkey.
Department of Otolaryngology-Head and Neck Surgery, School of Medicine, Akdeniz University, Dumlupınar Boulevard 07058 Campus, Antalya, Turkey.
Eur Arch Otorhinolaryngol. 2020 May;277(5):1467-1472. doi: 10.1007/s00405-020-05824-z. Epub 2020 Feb 3.
In the present study, we investigated the potential application of elastic light single-scattering spectroscopy (ELSSS) as a noninvasive, adjunctive tool to differentiate between malignant and benign oral lesions in vivo.
ELSSS spectra were acquired from 52 oral lesions of 47 patients prior to surgical biopsy using a single optical fiber probe. The sign of the spectral slope was used as a diagnostic parameter and was compared to the histopathology findings to obtain sensitivity and specificity of the ELSSS system in differentiating between benign and malignant tissues.
The sign of the spectral slope was positive for the benign tissues and negative for the malignant tissues. Nine malignant lesions and one high-grade dysplasia were correctly classified as cancerous. Six out of the ten low-grade dysplasia were correctly classified as cancerous, and four of them were misclassified as benign. Thirty benign lesions were correctly classified as benign, and two were misclassified as malignant. Our results indicate that the sign of the spectral slope enables the differentiation between malignant and benign oral lesions with a sensitivity of 80% and specificity of 94%.
ELSSS has the potential to be developed as an adjunctive screening tool in the noninvasive evaluation of oral lesions in vivo. This new diagnostic system may reduce the number of unnecessary biopsies.
本研究旨在探讨弹性光单次散射光谱（ELSSS）作为一种非侵入性的辅助工具，用于活体鉴别良恶性口腔病变的潜在应用。
使用单光纤探头，在手术活检前从 47 名患者的 52 个口腔病变中获取 ELSSS 光谱。光谱斜率的符号被用作诊断参数，并与组织病理学结果进行比较，以获得 ELSSS 系统在鉴别良恶性组织方面的敏感性和特异性。
良性组织的光谱斜率符号为正，恶性组织的光谱斜率符号为负。9 个恶性病变和 1 个高级别上皮内瘤变被正确分类为癌症。10 个低级别上皮内瘤变中有 6 个被正确分类为癌症，其中 4 个被错误分类为良性。30 个良性病变被正确分类为良性，其中 2 个被错误分类为恶性。我们的结果表明，光谱斜率的符号能够以 80%的敏感性和 94%的特异性区分良恶性口腔病变。
ELSSS 有潜力发展成为一种辅助性的筛选工具，用于活体评估口腔病变。这种新的诊断系统可能会减少不必要的活检数量。
