He Xiao-wei, Cao Hao-qiang, Xu Lu-ping, Zhou Jun, Xu Liu, Zhong Zhen-xiang, Lu Song-chun, Wu Wan-xin, Liu Ying-bin
Department of General Surgery, Jiaxing First Hospital, Jiaxing College, Jiaxing, Zhejiang, China.
Zhonghua Yi Xue Za Zhi. 2011 Jul 12;91(26):1852-5.
To establish the serial cell lines, derived from the same parental gallbladder cancer cell line GBC-SD, with highly metastatic potential via different routes and characterize their biological behaviors to understand the different metastasis mechanisms via lymph and blood.
The spleen-liver metastasis model and footpad-inguinal lymph node metastasis model were established. GBC-SD was injected into spleen or footpad of nude mice. Then the highly metastasized subpopulations via lymph and blood were isolated. Their differences in morphology, genetic background, proliferation, migration, invasion and adhesion were revealed by comparing the lymphatic-disseminating and hematogenous-disseminating subpopulations with parental cells.
The lymphatic-disseminating and hematogenous-disseminating subpopulations were successfully isolated and designated as GBC-SD/HL and GBC-SD/M3 respectively. They demonstrated the identical genetic background with GBC-SD. In comparison with parental cells, the hematogenous-disseminating subpopulation was morphologically characterized with epithelial-mesenchymal transition (EMT) while it was not shown in the lymphatic-disseminating subpopulation. Furthermore, the hematogenous-disseminating subpopulation showed the strongest migrating capacity but the lymphatic-disseminating subpopulation demonstrated a stronger invasive and adhesive ability.
The whole parental cell GBC-SD, hematogenous-metastasized subpopulation GBC-SD/M3 and lymphatic-disseminating subpopulation GBC-SD/HL is an ideal tool for metastatic mechanism study of gallbladder cancer. EMT plays an important role in hematogenous metastasis while lymphatic metastasis relies more on enhanced invasiveness and adhesion. It may be a target for interfering the lymphatic metastasis of gallbladder cancer.
通过不同途径建立源自同一亲本胆囊癌细胞系GBC-SD的具有高转移潜能的系列细胞系，并对其生物学行为进行表征，以了解经淋巴和血液的不同转移机制。
建立脾-肝转移模型和足垫-腹股沟淋巴结转移模型。将GBC-SD注入裸鼠的脾脏或足垫。然后分离经淋巴和血液的高转移亚群。通过将淋巴转移亚群和血行转移亚群与亲本细胞进行比较，揭示它们在形态、遗传背景、增殖、迁移、侵袭和黏附方面的差异。
成功分离出淋巴转移亚群和血行转移亚群，分别命名为GBC-SD/HL和GBC-SD/M3。它们与GBC-SD具有相同的遗传背景。与亲本细胞相比，血行转移亚群在形态上具有上皮-间质转化（EMT）特征，而淋巴转移亚群未表现出该特征。此外，血行转移亚群显示出最强的迁移能力，而淋巴转移亚群表现出更强的侵袭和黏附能力。
亲本细胞GBC-SD、血行转移亚群GBC-SD/M3和淋巴转移亚群GBC-SD/HL是研究胆囊癌转移机制的理想工具。EMT在血行转移中起重要作用，而淋巴转移更多地依赖于增强的侵袭性和黏附性。它可能是干扰胆囊癌淋巴转移的一个靶点。
