College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resources Reuse, Tongji University, Shanghai 200092, PR China.
Bioresour Technol. 2012 Jan;104:530-6. doi: 10.1016/j.biortech.2011.10.034. Epub 2011 Oct 28.
Considering that the surface properties of demulsifying cells correlate with their demulsification efficiency, the demulsifying bacteria Alcaligenes sp. S-XJ-1 with various surface properties were obtained using different vegetable oils as carbon sources. The results show that better performance was achieved with demulsifying bacteria S-XJ-1 possessing a relatively high cell surface hydrophobicity (CSH) and total unsaturated degree for the cell-wall bound fatty acids. There also appeared to be a correlation between the specific cell-wall bound fatty acid components of the bacteria, in terms of carbon chain length or degree of unsaturation, and either CSH or demulsification efficiency. The fatty acids attached to the cell wall were mainly composed of palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1), linoleic acid (C18:2) and linolenic acid (C18:3). C18:1 and C18:2 had a positive effect on the formation of CSH, while C18:0 and C18:3 had the opposite effect.
考虑到破乳细胞的表面性质与其破乳效率相关，使用不同的植物油作为碳源获得了具有不同表面性质的破乳细菌 Alcaligenes sp. S-XJ-1。结果表明，破乳细菌 S-XJ-1 具有较高的细胞表面疏水性（CSH）和细胞壁结合脂肪酸的总不饱和度时，表现出更好的性能。此外，细菌细胞壁结合脂肪酸的特定成分，无论是碳链长度还是不饱和度，都与 CSH 或破乳效率之间存在相关性。附着在细胞壁上的脂肪酸主要由棕榈酸（C16:0）、硬脂酸（C18:0）、油酸（C18:1）、亚油酸（C18:2）和亚麻酸（C18:3）组成。C18:1 和 C18:2 对 CSH 的形成有积极影响，而 C18:0 和 C18:3 则有相反的影响。
