College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Ministry of Education Key Laboratory of Yangtze River Water Environment, Tongji University, Shanghai 200092, China.
Bioresour Technol. 2013 Jul;139:257-64. doi: 10.1016/j.biortech.2013.04.043. Epub 2013 Apr 20.
The main goal of this work was to analyze the effect of surface substances on demulsifying capability of the demulsifying strain Alcaligenes sp. S-XJ-1. The demulsifying substances were successfully separated from the cell surface with dichloromethane-alkali treatment, and exhibited 67.5% of the demulsification ratio for water-in-kerosene emulsions at a dosage of 356mg/L. FT-IR, TLC and ESI-MS analysis confirmed the presence of a carbohydrate-protein-lipid complex in the demulsifying substances with the major molecular ions from mass-to-charge ratio (m/z) 165 to 814. After the substances separated, the cell morphology changed from aggregated to dispersed, and the concentration of cell surface functional groups decreased. Cell surface hydrophobicity and the ability of cell adhesion to hydrophobic surface of the treated cells was also reduced compared with original cell. It was proved that the demulsifying substances had a significant effect on cell surface properties and accordingly with demulsifying capability of Alcaligenes sp. S-XJ-1.
本工作的主要目的是分析表面物质对破乳菌株 Alcaligenes sp. S-XJ-1 破乳能力的影响。采用二氯甲烷-碱处理法从细胞表面成功分离出破乳物质，在 356mg/L 的用量下，对水包煤油乳液的破乳率达到 67.5%。FT-IR、TLC 和 ESI-MS 分析证实，破乳物质中存在一种碳水化合物-蛋白质-脂类复合物，其主要分子离子的质荷比（m/z）为 165 至 814。分离后，细胞形态由聚集变为分散，细胞表面官能团的浓度降低。与原始细胞相比，处理过的细胞的表面疏水性和对疏水表面的细胞粘附能力也降低。证明破乳物质对细胞表面特性有显著影响，从而影响了 Alcaligenes sp. S-XJ-1 的破乳能力。
