Sutphin George L, Olsen Brady A, Kennedy Brian K, Kaeberlein Matt
Department of Pathology and the Molecular and Cellular Biology Program, University of Washington, Seattle, WA, 98195-7470, USA,
Subcell Biochem. 2012;57:251-89. doi: 10.1007/978-94-007-2561-4_12.
In the past several decades the budding yeast Saccharomyces cerevisiae has emerged as a prominent model for aging research. The creation of a single-gene deletion collection covering the majority of open reading frames in the yeast genome and advances in genomic technologies have opened yeast research to genome-scale screens for a variety of phenotypes. A number of screens have been performed looking for genes that modify secondary age-associated phenotypes such as stress resistance or growth rate. More recently, moderate-throughput methods for measuring replicative life span and high-throughput methods for measuring chronological life span have allowed for the first unbiased screens aimed at directly identifying genes involved in determining yeast longevity. In this chapter we discuss large-scale life span studies performed in yeast and their implications for research related to the basic biology of aging.
在过去几十年中，出芽酵母酿酒酵母已成为衰老研究的一个重要模型。覆盖酵母基因组中大多数开放阅读框的单基因缺失文库的创建以及基因组技术的进步，使酵母研究能够针对各种表型进行全基因组规模的筛选。已经进行了多项筛选，以寻找修饰与年龄相关的次要表型（如抗逆性或生长速率）的基因。最近，用于测量复制寿命的中等通量方法和用于测量时序寿命的高通量方法，使得首次能够进行旨在直接鉴定参与决定酵母寿命的基因的无偏筛选。在本章中，我们将讨论在酵母中进行的大规模寿命研究及其对衰老基础生物学相关研究的意义。
