Department of Biochemistry and Biotechnology, Annamalai University, India.
Am J Nephrol. 2011;34(6):529-33. doi: 10.1159/000332042. Epub 2011 Nov 16.
BACKGROUND/AIMS: Soy protein improves renal function and prevents albuminuria in diabetic rats. This study investigates whether the renoprotective effect of soy protein is related to sustenance of basement membrane integrity.
Adult male albino rats were randomized into four groups and fed one of the following semi-synthetic diets consisting of corn starch (60%) and casein (20%; CCD), fructose (60%) and casein (20%; FCD), fructose (60%) and soy protein (20%; FSD), or corn starch (60%) and soy protein (20%; CSD). Plasma chemistry and renal changes were analyzed after 60 days.
FCD rats displayed metabolic derangements and renal ultrastructural changes. FSD rats showed reduction in type IV collagen, tissue inhibitor for matrix metallo-proteinase-2, vascular endothelial growth factor and tumor necrosis factor-α expression and improved matrix metallo-proteinase expression. Renal architecture was preserved in these rats.
Soy protein supplementation not only improved insulin sensitivity but also markedly attenuated renal basement membrane changes in fructose diet-fed rats. These findings provide evidence in support of the use of dietary soy protein in patients with diabetic kidney disease.
背景/目的：大豆蛋白可改善糖尿病大鼠的肾功能并预防白蛋白尿。本研究旨在探讨大豆蛋白的肾脏保护作用是否与维持基底膜完整性有关。
成年雄性白化大鼠随机分为四组，分别给予以下半合成饮食中的一种：玉米淀粉（60%）和酪蛋白（20%；CCD）、果糖（60%）和酪蛋白（20%；FCD）、果糖（60%）和大豆蛋白（20%；FSD）或玉米淀粉（60%）和大豆蛋白（20%；CSD）。60 天后分析血浆化学和肾脏变化。
FCD 大鼠表现出代谢紊乱和肾脏超微结构变化。FSD 大鼠的 IV 型胶原、基质金属蛋白酶抑制剂-2、血管内皮生长因子和肿瘤坏死因子-α的表达减少，基质金属蛋白酶的表达增加。这些大鼠的肾脏结构得到了保留。
大豆蛋白的补充不仅改善了胰岛素敏感性，而且显著减轻了果糖饮食喂养大鼠的肾脏基底膜变化。这些发现为在糖尿病肾病患者中使用膳食大豆蛋白提供了证据。
