Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD, USA.
Am J Clin Pathol. 2011 Dec;136(6):944-53. doi: 10.1309/AJCP09OTJOYAVZZK.
By using flow cytometry with markers for CD3, CD4, CD26, and CD7, we examined the blood samples of 109 patients for abnormal T cells: 69 patients with mycosis fungoides (MF)/Sézary syndrome (SS), 31 hospitalized control subjects, and 9 patients with inflammatory skin disease. T cells were identified as quantitatively abnormal (>15% CD26- or CD7- T cells) or phenotypically abnormal (CD26- or CD7- T cells with bright or dim CD3 or CD4 or bright CD7). Patients were followed for a median of 82 months, and abnormal T cells were correlated with diagnosis, clinical outcome, and other laboratory parameters. Abnormal T-cell populations were identified in 46% of patients with MF/SS (32/69) and correlated with disease extent. Quantitative abnormalities were more frequent than phenotypic abnormalities, and CD4+/CD26- T cells were more frequent than CD4+/CD7- T cells. CD26- T cells correlated better with disease extent than did CD7 -. Increasing numbers of abnormal T cells were associated with worsening disease. Flow cytometry provides valuable information for diagnosis, prognosis, and therapeutic efficacy in MF/SS.
我们使用流式细胞术检测 CD3、CD4、CD26 和 CD7 标志物，对 109 名患者的血液样本进行异常 T 细胞检测：69 名蕈样肉芽肿（MF）/Sézary 综合征（SS）患者、31 名住院对照患者和 9 名炎症性皮肤病患者。T 细胞被确定为数量异常（>15%的 CD26-或 CD7-T 细胞）或表型异常（CD26-或 CD7-T 细胞，具有明亮或暗淡的 CD3 或 CD4 或明亮的 CD7）。对患者进行中位数为 82 个月的随访，异常 T 细胞与诊断、临床结果和其他实验室参数相关。在 46%的 MF/SS 患者（32/69）中发现异常 T 细胞群，与疾病范围相关。定量异常比表型异常更常见，CD4+/CD26-T 细胞比 CD4+/CD7-T 细胞更常见。与 CD7-相比，CD26-T 细胞与疾病范围的相关性更好。异常 T 细胞数量的增加与疾病恶化相关。流式细胞术为 MF/SS 的诊断、预后和治疗效果提供了有价值的信息。
