Wood G S, Hong S R, Sasaki D T, Abel E A, Hoppe R T, Warnke R A, Morhenn V B
Department of Dermatology, Stanford University Medical Center, CA.
J Am Acad Dermatol. 1990 Apr;22(4):602-7. doi: 10.1016/0190-9622(90)70080-2.
Deficiencies of Leu-8 and CD7 antigens are exhibited by CD3+ T cells in the skin lesions of most patients with mycosis fungoides/Sézary syndrome. To determine whether these antigenic abnormalities are limited to involved skin, we studied Leu-8/CD7 expression in 21 skin lesions of mycosis fungoides/Sézary syndrome obtained from 16 patients and compared them with their peripheral blood leukocytes obtained concurrently. There was no correlation between Leu-8/CD7 values in skin lesions versus blood. Blood values were relatively uniform; most patients had 50% or greater of CD3+, Leu-8+ T cells and CD3+, CD7+ T cells. In contrast, skin values were highly heterogeneous; most patients lacked expression of Leu-8 or CD7 by the majority of lesional CD3+ T cells. Furthermore, Leu-8/CD7 antigen deficiency was present in lesional skin in one patient with mycosis fungoides but not in her concurrently sampled pityriasis lichenoides chronica or blood. These findings suggest that Leu-8/CD7 antigen deficiencies in skin lesions of mycosis fungoides/Sézary syndrome do not represent generalized antigenic abnormalities of CD3+ T cells in other body compartments and that within the skin, these deficiencies are disease specific within individual patients with more than one dermatosis. Comparative peripheral blood immunophenotyping of the patients with mycosis fungoides/Sézary syndrome and of the control subjects indicated that the control ranges of CD3+/Leu-8+ and CD3+/CD7+ T cells (33% or greater) extend lower than reported previously (60% or greater) and suggested that leukemic involvement in patients with mycosis fungoides/Sézary syndrome may correlate with percentages of CD3+, Leu8+ and/or CD3+, CD7+ T cells that fall below the revised control range.
大多数蕈样肉芽肿/赛塞里综合征患者皮肤病变中的CD3⁺ T细胞存在Leu-8和CD7抗原缺陷。为了确定这些抗原异常是否仅限于受累皮肤，我们研究了16例患者的21个蕈样肉芽肿/赛塞里综合征皮肤病变中的Leu-8/CD7表达，并将其与同时采集的外周血白细胞进行比较。皮肤病变与血液中的Leu-8/CD7值之间无相关性。血液值相对一致；大多数患者的CD3⁺、Leu-8⁺ T细胞和CD3⁺、CD7⁺ T细胞占比达50%或更高。相比之下，皮肤值高度异质性；大多数患者的病变CD3⁺ T细胞多数缺乏Leu-8或CD7表达。此外，1例蕈样肉芽肿患者的病变皮肤存在Leu-8/CD7抗原缺陷，但同时采集的慢性苔藓样糠疹或血液中未出现。这些发现表明，蕈样肉芽肿/赛塞里综合征皮肤病变中的Leu-8/CD7抗原缺陷并不代表其他身体部位CD3⁺ T细胞的全身性抗原异常，且在皮肤内，这些缺陷在患有多种皮肤病的个体患者中具有疾病特异性。蕈样肉芽肿/赛塞里综合征患者与对照受试者的外周血免疫表型比较表明，CD3⁺/Leu-8⁺和CD3⁺/CD7⁺ T细胞的对照范围（33%或更高）低于先前报道（60%或更高），提示蕈样肉芽肿/赛塞里综合征患者的白血病累及可能与低于修订后对照范围的CD3⁺、Leu8⁺和/或CD3⁺、CD7⁺ T细胞百分比相关。
