Li Minyu, Han Baoqin, Liu Wanshun
College of Marine Life Sciences, Ocean University of China, Qingdao, People's Republic of China.
J Mater Sci Mater Med. 2011 Dec;22(12):2745-55. doi: 10.1007/s10856-011-4455-8. Epub 2011 Nov 18.
A novel drug loaded membrane made of N-succinyl-hydroxyethyl chitosan and mitomycin C was used as an implant for glaucoma filtering surgery. The characteristics of the membrane, such as FTIR, equilibrium water content, swelling ratio, permeability, and drug release in vitro were determined. The L929 fibroblast inhibition of drug loaded membranes was compared to hydroxyethyl chitosan film and blank control, detecting by MTT. The biodegradability and biocompatibility were evaluated by implanting membranes into the subcutaneous tissue and muscle of rats. FTIR indicated mitomycin C was introduced. The experimental results indicated the drug loaded membrane was effective on the swelling property, permeability, and drug release in vitro. Cell culture experimental results demonstrated that the destination membrane inhibited fibroblast proliferation. In vivo, the membranes showed bioabsorption and biocompatibility. The experimental results provide a theoretical basis for the future development of the drug loaded membrane as an implant for increasing the success rate of filtering surgery.
一种由N-琥珀酰-羟乙基壳聚糖和丝裂霉素C制成的新型载药膜被用作青光眼滤过手术的植入物。测定了该膜的特性，如傅里叶变换红外光谱（FTIR）、平衡含水量、溶胀率、渗透率和体外药物释放。通过MTT检测，将载药膜对L929成纤维细胞的抑制作用与羟乙基壳聚糖膜和空白对照进行比较。通过将膜植入大鼠皮下组织和肌肉来评估其生物降解性和生物相容性。FTIR表明引入了丝裂霉素C。实验结果表明，载药膜在体外的溶胀性能、渗透率和药物释放方面是有效的。细胞培养实验结果表明，目标膜抑制成纤维细胞增殖。在体内，这些膜表现出生物吸收性和生物相容性。实验结果为载药膜作为一种提高滤过手术成功率的植入物的未来发展提供了理论依据。
