College of Marine Life Sciences, Ocean University of China, Shandong, Qingdao, PR China.
Affiliated Hospital of Heze Medical College, Heze, Shandong, PR China.
J Mater Sci Mater Med. 2017 Aug 22;28(10):149. doi: 10.1007/s10856-017-5954-z.
Hydroxyethyl chitosan (HECTS) is a critical derivative of chitosan that has been widely used as biomedical materials due to great water-solubility and excellent biocompatibility. Here, photosensitive hydroxyethyl chitosan was synthesized by introducing azide group on NH2 of HECTS (HECTS-AZ), afterwards FTIR and H NMR spectra were detected to confirm the formation of HECTS-AZ. The solution of HECTS-AZ can achieve a sol-gel transition through UV irradiation for 30 s. The evaluation of biocompability and biodegradability in vivo was conducted in rats, visual and pathological examinations exhibited the HECTS-AZ has excellent biocompability and degradation time of the hydrogel is more than 14 weeks. Furthermore, HECTS-AZ hydrogel as an ocular drug delivery system loading heparin was prepared to implant under sclera of rabbit after glaucoma filtration surgery (GFS). The experimental results demonstrated the heparin loaded hydrogel can effectively maintain filtration bleb and lowing intraocular pressure (IOP) after GFS for prolonged time. Besides, obvious inflammatory reactions and side effects have not been observed in ocular during the experimental period. In conclusion, the HECTS-AZ hydrogel is a potential drug delivery device for the treatment of glaucoma and other ocular diseases.
羟乙基壳聚糖（HECTS）是壳聚糖的重要衍生物，由于其良好的水溶性和出色的生物相容性，已被广泛用作生物医学材料。在这里，通过在 HECTS 的 NH2 上引入叠氮基团来合成光敏羟乙基壳聚糖（HECTS-AZ），随后通过傅里叶变换红外光谱（FTIR）和核磁共振（1H NMR）谱进行检测，以确认 HECTS-AZ 的形成。HECTS-AZ 的溶液通过 30 秒的紫外光照射即可实现溶胶-凝胶转变。通过在大鼠体内进行生物相容性和体内降解性评价，肉眼和组织病理学检查显示 HECTS-AZ 具有出色的生物相容性，水凝胶的降解时间超过 14 周。此外，将 HECTS-AZ 水凝胶作为载肝素的眼用药物传递系统，在青光眼滤过手术后（GFS）植入兔眼巩膜下。实验结果表明，载肝素的水凝胶可以在 GFS 后有效维持滤过泡并降低眼内压（IOP），并延长时间。此外，在实验期间，眼部未观察到明显的炎症反应和副作用。总之，HECTS-AZ 水凝胶是治疗青光眼和其他眼部疾病的一种有潜力的药物输送装置。
