Department of Environmental and Agricultural Chemistry, Università Cattolica del Sacro Cuore, Via Emilia Parmense 84, I-29122 Piacenza, Italy.
J Sci Food Agric. 2012 Apr;92(6):1297-303. doi: 10.1002/jsfa.4698. Epub 2011 Nov 14.
The tomato fruit is a dietary source of carotenoids, bioactive antioxidant compounds that play an important role in the prevention of degenerative diseases. Several extraction and detection techniques regarding carotenoids in tomatoes can be found in the literature, mainly based on high-performance liquid chromatography separation and ultraviolet-visible detection.
The best extraction conditions and tandem mass spectrometry (MS) analysis were evaluated: lycopene and β-carotene were extracted in a cyclohexane/ethyl acetate mixture without the addition of antioxidants, next separated by liquid chromatography on a C(18) column and then determined through electrospray tandem MS. Ionic suppression by the matrix in negative ionisation mode did not allow the analysis of extracts, hence the positive ionisation mode was chosen. Validation parameters demonstrated the suitability for purpose of the analytical method: accuracy, precision, linearity and detection limits were adequate. The method was finally applied to different tomato samples, and differences could be easily highlighted.
The method was simple, fast and appropriate for the purpose of analysing lycopene and β-carotene in tomatoes.
番茄果实是类胡萝卜素的膳食来源，类胡萝卜素是具有生物活性的抗氧化化合物，在预防退行性疾病方面发挥着重要作用。文献中可以找到几种关于番茄中类胡萝卜素的提取和检测技术，主要基于高效液相色谱分离和紫外-可见检测。
评估了最佳的提取条件和串联质谱（MS）分析：番茄红素和β-胡萝卜素在没有添加抗氧化剂的环己烷/乙酸乙酯混合物中提取，然后通过 C（18）柱上的液相色谱分离，然后通过电喷雾串联 MS 进行测定。负离子模式下基质的离子抑制不允许对提取物进行分析，因此选择了正离子化模式。验证参数表明该分析方法符合目的要求：准确度、精密度、线性和检测限都足够。该方法最终应用于不同的番茄样品，可以很容易地突出显示差异。
该方法简单、快速，适用于分析番茄中的番茄红素和β-胡萝卜素。
