Department of Chemistry, Lleida University, Alcalde Rovira Roure 191, Lleida 25198, Spain.
Molecules. 2012 Sep 21;17(9):11255-68. doi: 10.3390/molecules170911255.
We performed a number of tests with the aim to develop an effective extraction method for the analysis of carotenoid content in maize seed. Mixtures of methanol-ethyl acetate (6:4, v/v) and methanol-tetrahydrofuran (1:1, v/v) were the most effective solvent systems for carotenoid extraction from maize endosperm under the conditions assayed. In addition, we also addressed sample preparation prior to the analysis of carotenoids by liquid chromatography (LC). The LC response of extracted carotenoids and standards in several solvents was evaluated and results were related to the degree of solubility of these pigments. Three key factors were found to be important when selecting a suitable injection solvent: compatibility between the mobile phase and injection solvent, carotenoid polarity and content in the matrix.
我们进行了一系列的测试，旨在开发一种有效的提取方法，用于分析玉米种子中的类胡萝卜素含量。在测试条件下，甲醇-乙酸乙酯（6:4，v/v）和甲醇-四氢呋喃（1:1，v/v）的混合物是从玉米胚乳中提取类胡萝卜素最有效的溶剂体系。此外，我们还针对通过液相色谱（LC）分析类胡萝卜素之前的样品制备进行了讨论。评估了在几种溶剂中提取的类胡萝卜素和标准品的 LC 响应，并将结果与这些色素的溶解度程度相关联。在选择合适的进样溶剂时，发现有三个关键因素非常重要：流动相和进样溶剂的兼容性、类胡萝卜素的极性以及基质中的含量。
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