Rinkevich B, Weissman I L
Israel Oceanographic and Limnological Research, Haifa.
Eur J Immunol. 1990 Aug;20(8):1775-9. doi: 10.1002/eji.1830200823.
It has been previously shown that mixed-cell chimeras may be established between different colonies of the compound tunicate Botryllus schlosseri given that they share one allele in common on the fusibility locus. However, one of the partners in each chimera is often resorbed. Here we tested for the existence of a memory component to this response, as measured by the accelerated interval to resorption of a second set of semiallogeneic colonies. Eight genetically unrelated colonies gave rise to eighteen chimeras. After a complete resorption of the "inferior" partners in these chimeras, secondary and tertiary sets of chimeras were established with some of them by fusion of the "superior" with naive subclones of the "inferior" partners. It is shown here that (a) when multiple chimeras are prepared using the same pair of colonies, subclones of only one of the partners are resorbed; (b) high variation in the time for resorption is recorded when several chimeras are prepared from any specific pair of colonies, and (c) the time for resorption is not related to the number of vessels connecting between the partners in the chimera, nor to their relative body sizes. Allospecific memory is not documented here and reasons for its absence are discussed.
先前的研究表明，鉴于复合被囊动物柄海鞘不同群体在融合位点共享一个等位基因，它们之间可能建立混合细胞嵌合体。然而，每个嵌合体中的一方通常会被吸收。在此，我们测试了这种反应中是否存在记忆成分，通过测量第二组半同种异体群体被吸收的加速间隔来衡量。八个遗传上不相关的群体产生了18个嵌合体。在这些嵌合体中“劣势”一方完全被吸收后，通过“优势”一方与“劣势”一方的原始亚克隆融合，建立了二级和三级嵌合体。结果表明：（a）当使用同一对群体制备多个嵌合体时，只有一方的亚克隆会被吸收；（b）从任何特定的一对群体制备多个嵌合体时，吸收时间存在很大差异；（c）吸收时间与嵌合体中双方连接的血管数量及其相对体型均无关。本文未记录同种异体记忆，并讨论了其不存在的原因。
