Sabbadin A, Astorri C
Dipartimento di Biologia, Università di Padova, Italy.
Dev Comp Immunol. 1988 Fall;12(4):737-47. doi: 10.1016/0145-305x(88)90049-3.
Chimeras of B. schlosseri were prepared by pairwise combination of colonies sharing one allele at the fusibility gene locus (AD = AC chimeras). A frequent resorption of one of the partners was observed and the resorption time was shown to be significantly correlated with the relative size, the resorbing partner being usually the larger. Both in the whole chimeras, AD = AC, and in the separated partners, (AD)AC, the fusibility of AC was frequently altered. In AD = AC the fusion did not prevent entirely AC fusion with BC. The fusion frequency with BC was significantly higher than with BD and significantly higher in (AD)AC than in AD = AC. Repeated rejections with BC or both BC and BD, repeated fusions with BD, simultaneous or successive fusions and rejections especially with BD, over a period of several months, indicated a long lasting competitive interaction of AD and AC in (AD)AC chimeras. The persistence in these chimeras of the AD cell population was also confirmed by the chimeric electrophoretic pattern of PGI in most of them.
通过在融合性基因位点共享一个等位基因的菌落进行两两组合，制备了施氏苔藓虫的嵌合体（AD = AC嵌合体）。观察到一个伙伴经常会被吸收，且吸收时间与相对大小显著相关，吸收的伙伴通常是较大的那个。在整个AD = AC嵌合体以及分离的伙伴（AD）AC中，AC的融合性经常发生改变。在AD = AC中，融合并不能完全阻止AC与BC的融合。与BC的融合频率显著高于与BD的融合频率，并且在（AD）AC中比在AD = AC中显著更高。在几个月的时间里，对BC或同时对BC和BD进行反复排斥，对BD进行反复融合，同时或相继进行融合和排斥，尤其是与BD，这表明在（AD）AC嵌合体中AD和AC之间存在长期的竞争性相互作用。大多数嵌合体中PGI的嵌合电泳图谱也证实了AD细胞群体在这些嵌合体中的持久性。
