Li Chunnan, Sun Jiaming, Zhang Hui
The Research and Development Center of Traditional Chinese Medicine and Bioengineering, Changchun University of Traditional Chinese Medicine, Changchun 130117, China.
Zhongguo Zhong Yao Za Zhi. 2011 Aug;36(16):2183-6.
To study collagen structure of the traditional Chinese medicine elephant skin and the proposed alternatives such as pig skin, fish scale, and antioxidant activity.
Orthogonal experimental design method was employed to determine the optimal extraction condition of collagen from the elephant skin, and the structure and content of collagen of proposed alternatives were compared, their scavenging ability were determined by salicylic acid.
Collagen extracted from elephant skin with the optimal conditions was the structural integrity and good quality first time, and collagen structure of the elephant skin was similar to the proposed alternatives. Free radical scavenging capacity of collagen, values of IC50, were 0.51 g x L(-1) of elephant skin, 0.60 g x L(-1) of pig skin and 0.42 g x L(-1) of fish scale.
By comparing and identification of proteins that the collagen of elephant skin is type I collagen, with a strong antioxidant capacity, is the active ingredients of elephant skin. It provides a further study of alternatives as an important reference.
研究中药象皮及其替代物如猪皮、鱼鳞的胶原蛋白结构和抗氧化活性。
采用正交试验设计法确定象皮胶原蛋白的最佳提取条件，比较替代物胶原蛋白的结构和含量，用水杨酸法测定其清除自由基能力。
在最佳条件下从象皮中提取的胶原蛋白首次获得结构完整、质量良好的产品，象皮的胶原蛋白结构与替代物相似。胶原蛋白的自由基清除能力，半数抑制浓度（IC50）值分别为：象皮0.51 g·L⁻¹、猪皮0.60 g·L⁻¹、鱼鳞0.42 g·L⁻¹。
通过蛋白质比较鉴定，象皮胶原蛋白为Ⅰ型胶原蛋白，具有较强抗氧化能力，是象皮的活性成分。为进一步研究替代物提供了重要参考。
