Zhejiang Academy of Medical Science, Hangzhou 310013, China.
J Sci Food Agric. 2010 Mar 15;90(4):635-40. doi: 10.1002/jsfa.3861.
Fish skin, a by-product of the food industry, contains a large amount of collagen. However, only a small proportion of fish skin is used in the production of leather materials and animal feedstuffs, most of it being discarded. The aims of this study were to prepare peptides from Alaska pollack (Theragra chalcogramma) skin by enzymatic hydrolysis and to evaluate the antioxidant activity of the resulting hydrolysate.
Protamex was the most efficient enzyme for preparing antioxidant peptides from Alaska pollack skin. The optimal hydrolysis conditions were as follows: hydrolysis time 8 h; enzyme/substrate ratio 2:1000; skin/water ratio 1:6; temperature 55 degrees C; pH 6.0. Under these conditions the highest yield of peptides was 83.44%, with 85.95% of the hydrolysate being mainly composed of oligopeptides with molecular weights ranging from 180 to 1000 Da. The hydrolysate showed 2,2-diphenyl-1-picrylhydrazyl radical-scavenging activity, with an IC(50) value of 2.5 mg mL(-1), and its reducing power was 0.14 at 1 mg mL(-1), 53.8% of that of reduced glutathione at the same concentration.
This study demonstrated that the hydrolysate of Alaska pollack skin was mainly composed of oligopeptides with two to eight amino acid residues and possessed antioxidant activity.
鱼皮是食品工业的副产品，含有大量的胶原蛋白。然而，只有一小部分鱼皮用于生产皮革材料和动物饲料，大部分都被丢弃了。本研究的目的是通过酶解从阿拉斯加狭鳕鱼皮中制备肽，并评价所得水解产物的抗氧化活性。
Protamex 是从阿拉斯加狭鳕鱼皮中制备抗氧化肽最有效的酶。最佳水解条件为：水解时间 8 h；酶/底物比 2:1000；皮/水比 1:6；温度 55 摄氏度；pH 值 6.0。在此条件下，肽的最高得率为 83.44%，水解产物中 85.95%主要由分子量在 180 至 1000 Da 之间的寡肽组成。该水解产物具有 2,2-二苯基-1-苦基肼自由基清除活性，IC50 值为 2.5 mg mL(-1)，在 1 mg mL(-1)时的还原力为 0.14，与相同浓度的还原型谷胱甘肽相比，还原力为 53.8%。
本研究表明，阿拉斯加狭鳕鱼皮的水解产物主要由具有二至八个氨基酸残基的寡肽组成，具有抗氧化活性。
