高效毛细管电泳-二极管阵列检测法同时测定地耳草中6种黄酮类成分
[Simultaneous determination of six flavonoids in Hyperici Japonici Herba by HPCE-DAD].
作者信息
Han Ke, Liu Xunhang, Wu Qinan, Li Junsong, Cai Baochang, Wang Lijuan, Fu Xingsheng, Lu Aiping
机构信息
Pharmnacology College of Nanjing University of Chinese Medicine, Nanjing 210046, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2011 Aug;36(16):2219-25.
OBJECTIVE
To establish a high performance capillary electrophoresis method with diode array detection (HPCE-DAD) for simultaneous determination of rutin, isoquercitrin, hyperoside, quercitrin, kaempferol and quercetin in Hyperici Japonici Herba.
METHOD
Based on the mode of capillary zone electrophoresis, 40 mmol x L(-1) borax was used as buffer solution (pH 8.62), uncoated fused silica capillary (56 cm x 64.5 cm x 75 microm) was used, separation voltage was 25 kV, detection wavelength was at 206 nm, column temperature was maintained at 25 degrees C, and sample was injected at 50 mbar, 8 s.
RESULT
Six flavonoids showed good linearity (r > 0.9953) in the range of the tested concentration, the average recoveries of the method were between 98.8%-102.9%.
CONCLUSION
The method is simple, accurate and reproducible, and can be used for quality control of Hyperici Japonici Herba.
目的
建立一种采用二极管阵列检测的高效毛细管电泳法（HPCE-DAD），用于同时测定地耳草中芦丁、异槲皮苷、金丝桃苷、槲皮苷、山柰酚和槲皮素的含量。
方法
基于毛细管区带电泳模式，采用40 mmol·L⁻¹硼砂作为缓冲溶液（pH 8.62），使用未涂层熔融石英毛细管（56 cm×64.5 cm×75 μm），分离电压为25 kV，检测波长为206 nm，柱温保持在25℃，进样压力为50 mbar，进样时间为8 s。
结果
6种黄酮类化合物在测试浓度范围内呈现良好的线性关系（r＞0.9953），该方法的平均回收率在98.8% - 102.9%之间。
结论
该方法简便、准确、重现性好，可用于地耳草的质量控制。
Zotero 插件