Wang Jianhong, Lu Fuer, Dong Hui, Gong Yanlin, Xu Lijun, Zou Xin, Chen Guang
Institute of Integrative Traditional Chinese & Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, China.
Zhongguo Zhong Yao Za Zhi. 2011 Aug;36(16):2271-6.
To study the effects of different compatibility proportion of Jiaotai pills on treating type 2 diabetes mellitus (T2DM) in rats.
The model of type 2 diabetes mellitus in rats was established by injecting streptozotocin from tail vein and feeding with high fat and high caloric diet. Diabetic rats were randomly divided into model group, Jiaotai pill 1 group (Coptidis Rhizoma-cinnamon 2: 1), Jiaotai pill 2 group (Coptidis Rhizoma-cinnamon 4: 1), Jiaotai pill 3 group (Coptidis Rhizoma-cinnamon 10: 1) and metformin group. Rats in different treatment groups were given by corresponding therapy from gastric tube. Meanwhile normal control group was another set. Body weight, oral glucose tolerance test (OGTT), blood lipid level including total cholesterol (TC), triglyceride (TG), high density lipoprotein-cholesterol (HDL-C) and low density lipoprotein-cholesterol (LDL-C), plasma levels of free fatty acid (FFA) and adiponectin, plasma liver enzymes activity(ALT, AST, AKP, gamma-GT) and pathological results of liver tissue were determined after eight weeks.
Body weight, fasting plasma glucose (FPG), postpradial plasma glucose at one hour (PG-1 h), postpradial blood glucose at two hour (PG-2 h), plasma levels of TC, TG, LDL-C, FFA and liver enzymes activity were all increased in rats of model group compared with those in normal control group. Plasma levels of HDL-C and adiponectin were decreased in model group (P < 0.01). Fatty degeneration of hepatocytes was apparent in liver tissues in rats of model group. Compared with model group results of OGTT, blood lipid levels and liver enzymes activity were improved while levels of HDL-C and adiponectin were increased in rats of different treatment groups (P < 0.05 or P < 0.01). Meanwhile fatty degeneration of hepatocytes was improved in liver tissues in rats of different treatment groups. Compared with metformin group, plasma level of HDL-C was elevated while AKP and gamma-GT were decreased significantly in rats of Jiaotai pill 1 group (P < 0.05), gamma-GT level was decreased significantly in rats of Jiaotai pill 2 group (P < 0.05), AST, AKP and gamma-GT levels were decreased significantly in rats of Jiaotai pill 3 group (P < 0.05). Compared with Jiaotai Pill 1 group, plasma levels of HDL-C was decreased while AKP levels was elevated significantly in rats of Jiaotai pill 2 group, but HDL-C was decreased in rats of Jiaotai pill 3 group (P < 0.05).
It is suggested that different compatibility proportion of Jiaotai pills are effective on treating type 2 diabetes mellitus in rats. The effect of Jiaotai pill 1 group is better than that of other therapy groups.
研究不同配伍比例交泰丸对大鼠2型糖尿病（T2DM）的治疗作用。
采用尾静脉注射链脲佐菌素并给予高脂高糖饮食的方法建立大鼠2型糖尿病模型。将糖尿病大鼠随机分为模型组、交泰丸1组（黄连-肉桂2∶1）、交泰丸2组（黄连-肉桂4∶1）、交泰丸3组（黄连-肉桂10∶1）和二甲双胍组。不同治疗组大鼠经胃管给予相应治疗。同时另设正常对照组。8周后测定大鼠体重、口服葡萄糖耐量试验（OGTT）、血脂水平包括总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）和低密度脂蛋白胆固醇（LDL-C）、血浆游离脂肪酸（FFA）和脂联素水平、血浆肝酶活性（ALT、AST、AKP、γ-GT）以及肝组织病理结果。
与正常对照组相比，模型组大鼠体重、空腹血糖（FPG）、餐后1小时血糖（PG-1 h）、餐后2小时血糖（PG-2 h）、血浆TC、TG、LDL-C、FFA水平及肝酶活性均升高。模型组血浆HDL-C和脂联素水平降低（P<0.01）。模型组大鼠肝组织可见明显的肝细胞脂肪变性。与模型组相比，不同治疗组大鼠OGTT、血脂水平及肝酶活性均有改善，同时HDL-C和脂联素水平升高（P<0.05或P<0.01）。不同治疗组大鼠肝组织肝细胞脂肪变性也有所改善。与二甲双胍组相比，交泰丸1组大鼠血浆HDL-C水平升高，AKP和γ-GT水平显著降低（P<0.05）；交泰丸2组大鼠γ-GT水平显著降低（P<0.05）；交泰丸3组大鼠AST、AKP和γ-GT水平显著降低（P<0.05）。与交泰丸1组相比，交泰丸2组大鼠血浆HDL-C水平降低，AKP水平显著升高；交泰丸3组大鼠HDL-C水平降低（P<0.05）。
不同配伍比例的交泰丸对大鼠2型糖尿病有治疗作用。交泰丸1组疗效优于其他治疗组。
