Xu Xiao-hong, Xie Zheng-xin, Ma Rong, Zhang Wei-qin, Li Qi-fa
Hafei Women & Children's Healthcare Hospital, Anhui, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2011 Aug;25(4):298-300.
To study the relationship between thinprep cytologic test and the types of human papilloma virus (HPV) infection in cervical precancerous lesion screening.
To perform high-risk HPV types test in 1375 samples. Choose 256 positive samples to take thinprep cytologic test (TCT) and directed biopsies under colposcopy. Adopting two-channels real time PCR to genotype and quantify eight high risk HPV DNA (high risk types: HPV 16, 18, 45, 31; intermediate risk types: HPV 33, 52, 58, 67).
There are 256 positive samples in High risk HPV DNA test (18.62%). WNL rate for TCT is 16.41% (42/256), ASCUS and above rate for TCT is 83.59% (214/256). There is no statistically significant difference in the viral loads of HPV infection rate between the TCT negative patients and positive patients (P > 0.5). Positive correspondence rate for TCT and biopsy are 92.86% (39/42), 81.36% (48/59), 85.19% (23/27) and 9/10.
High-risk HPV types checking combined with TCT and biopsy can raise positive rate significantly. It should be used as a reliable method for early diagnosis in cervical cancer and CIN screening.
研究液基薄层细胞学检测与宫颈癌前病变筛查中人类乳头瘤病毒（HPV）感染类型之间的关系。
对1375份样本进行高危型HPV检测。选取256份阳性样本进行液基薄层细胞学检测（TCT）及阴道镜下直接活检。采用双通道实时荧光定量PCR对8种高危型HPV DNA进行基因分型及定量分析（高危型：HPV 16、18、45、31；中危型：HPV 33、52、58、67）。
高危型HPV DNA检测阳性样本256份（18.62%）。TCT结果正常率为16.41%（42/256），TCT结果为非典型鳞状细胞及以上者占83.59%（214/256）。TCT阴性患者与阳性患者HPV感染率的病毒载量差异无统计学意义（P＞0.5）。TCT与活检结果的阳性符合率分别为92.86%（39/42）、81.36%（48/59）、85.19%（23/27）和9/10。
高危型HPV检测联合TCT及活检可显著提高阳性率。应作为宫颈癌及宫颈上皮内瘤变筛查早期诊断的可靠方法。
