Li Xiao, Qu Yanyan, Zhang Piqiao, Zhang Jin, Zhang Lihua, Huang Daliang, Zhang Yukui
Dalian Institute of Chemical Physics, CAS Key Laboratory of Separation Science for Analytical Chemistry, Chinese Academy of Sciences, Dalian 116023, China.
Se Pu. 2011 Jul;29(7):673-6.
Nine representative fish species from Bohai Bay were identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and lab-on-a-chip technology. The nine fish species were Pseudosciaena polyactis, Lateolabrax japonicus, Scomberomorus niphonius, Pneumatophorus japonicus, Pseudopleuronectes yokohamae, Collichthys lucidus, Sebastes schlegeli, Cleisthenes herzensteini and Saurida elongata. The 464 bp fragment of mitochondrial cytochrome b gene was amplified and further digested by Dde I , Hae III and Nla III. The digested DNA fragments were analyzed by microfluidic capillary electrophoresis. The nine fish species were fully discriminated. The results demonstrated that the proposed method is efficient, precise and fast in fish species identification.
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和芯片实验室技术鉴定了渤海湾的9种代表性鱼类。这9种鱼类分别是小黄鱼、日本鲈鱼、日本鲭鱼、日本鲐鱼、横滨黄盖鲽、棘头梅童鱼、许氏平鲉、赫氏银鲈和长蛇鲻。扩增线粒体细胞色素b基因的464 bp片段，并用Dde I、Hae III和Nla III进一步消化。通过微流控毛细管电泳分析消化后的DNA片段。这9种鱼类得到了完全鉴别。结果表明，该方法在鱼类物种鉴定中高效、准确、快速。
