Department of Chemistry, East China Normal University, Shanghai, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Dec 15;879(32):3818-22. doi: 10.1016/j.jchromb.2011.10.026. Epub 2011 Oct 29.
A sensitive and low-cost analytical method has been developed to determine 8-hydroxy-2'-deoxyguanosine (8-OHdG) and 8-nitroguanine (8-NO(2)Gua) based on capillary electrophoresis with amperometric detection (CE-AD) after solid phase extraction (SPE). Under optimized condition, these two markers were well separated from other components coexisting in urine, exhibiting a linear calibration over the concentration range of 0.1-50.0 μg/mL with the detection limits ranging from 0.02 to 0.06 μg/mL. The relative standard deviations (RSDs) were in the range of 0.1-2.1% for peak area, 0.1-1.5% for migration time, respectively. The average recovery and RSD were within the range of 100.0-108.0% and 0.1-1.7%, respectively. It was found that the urinary contents of 8-OHdG and 8-NO(2)Gua in cancer patients were significantly higher than those in healthy ones.
建立了一种基于固相萃取-毛细管电泳安培检测的灵敏、低成本分析方法，用于测定尿液中 8-羟基-2'-脱氧鸟苷（8-OHdG）和 8-硝基鸟嘌呤（8-NO2Gua）。在最佳条件下，这两种标志物能与尿液中其他共存物质很好地分离，在 0.1-50.0μg/mL 浓度范围内呈线性，检测限为 0.02-0.06μg/mL。峰面积和迁移时间的相对标准偏差（RSD）分别在 0.1-2.1%和 0.1-1.5%范围内。平均回收率和 RSD 分别在 100.0-108.0%和 0.1-1.7%范围内。结果发现，癌症患者尿液中的 8-OHdG 和 8-NO2Gua 含量明显高于健康人。
