Bider D, Pariente C, Dor J, Zolti M, Mashiach S, Ben-Rafael Z
Chaim Sheba Medical Center, Tel Aviv University, Tel Hashomer, Israel.
Fertil Steril. 1990 Oct;54(4):597-600. doi: 10.1016/s0015-0282(16)53814-x.
The effect of dexamethasone (DEX) (9 alpha-Fluro-16 alpha-methyl prednisolone) on secretion of steroids by human granulosa luteinized cells was studied by culturing cells from mature follicles of women with polycystic ovarian disease and treated for infertility in the in vitro fertilization program. Patients were treated with DEX 0.5 mg/d until the day of human chorionic gonadotropin administration. The cells were cultured for 24 hours in the presence of androstenedione (10(-7)M). After incubating for 24 hours, the medium was replaced and the cells were incubated for an additional 24 hours. The medium was then harvested and assayed for estradiol (E2) and progesterone (P). Results were compared with those of a control group who was not treated with DEX. Estradiol production by cells was significantly lower in the study group treated with DEX. Progesterone production was not influenced by DEX. Follicular fluid levels, E2, and androgens did not vary with DEX treatment, whereas cortisol levels markedly decreased and P levels increased with the treatment. These findings suggest that glucocorticosteroids can directly influence granulosa luteinized cell function.
通过培养多囊卵巢疾病患者成熟卵泡的细胞，并在体外受精程序中对其进行不育治疗，研究了地塞米松（DEX）（9α-氟-16α-甲基泼尼松龙）对人颗粒黄体化细胞类固醇分泌的影响。患者接受0.5mg/d的DEX治疗，直至注射人绒毛膜促性腺激素当天。细胞在雄烯二酮（10⁻⁷M）存在的情况下培养24小时。孵育24小时后，更换培养基，细胞再孵育24小时。然后收集培养基，检测雌二醇（E2）和孕酮（P）。将结果与未接受DEX治疗的对照组进行比较。接受DEX治疗的研究组细胞产生的雌二醇明显较低。孕酮的产生不受DEX影响。卵泡液水平、E2和雄激素不受DEX治疗的影响，而皮质醇水平随着治疗明显降低，P水平升高。这些发现表明糖皮质激素可直接影响颗粒黄体化细胞功能。
