Departamento de Microbiología, Facultad de Farmacia, Universidad de Granada, Campus de Cartuja s/n, 18071 Granada, Spain.
J Biosci Bioeng. 2012 Feb;113(2):179-84. doi: 10.1016/j.jbiosc.2011.10.010. Epub 2011 Nov 17.
Various microbial polymers, namely xanthan gum, gellan gum, pullulan gum and jamilan, were tested as a suitable encapsulating material for Lactobacillus plantarum CRL 1815 and Lactobacillus rhamnosus ATCC 53103. Resulting capsules were also studied for their pH and simulated gastrointestinal conditions tolerance. The morphology of the microcapsules was studied using scanning electron microscopy. pH tolerance was tested at pH 2.0, 3.5, 5.0 and 6.5 over a 6h incubation period. Simulated gastrointestinal conditions were assayed with simulated gastric and pancreatic juices and simulated bile over a 24h incubation period. Suspensions of probiotic organisms were used as a control. The results from encapsulation with microbial polymers indicate that mixtures of 1% xanthan gum with 0.75% gellan gum and 1% jamilan with 1% gellan gum were the most suitable for microencapsulation. Results for the pH tolerance tests showed no improvement in the viability of cells in relation to the control, except for pH 2.0 where lactobacilli encapsulated in xanthan:gellan gum (1%:0.75%) prolonged their viability by 6h exposure. Xanthan:gellan gum (1%:0.75%) was the most effective of the encapsulating materials tested in protecting L. plantarum and L. rhamnosus against simulated bile, improving its viability in 1-2 logCFU when compared with control. The results of this study suggest that microbial polymers are an interesting source of encapsulating material that should be taken into account for prospective studies of probiotic encapsulation for oral delivery applications.
各种微生物聚合物，即黄原胶、结冷胶、普鲁兰多糖和瓜尔胶，被测试作为植物乳杆菌 CRL 1815 和鼠李糖乳杆菌 ATCC 53103 的合适包埋材料。还研究了所得胶囊在 pH 值和模拟胃肠道条件下的耐受性。使用扫描电子显微镜研究微胶囊的形态。在 pH 2.0、3.5、5.0 和 6.5 下进行 6 小时孵育，测试 pH 值耐受性。用模拟胃液和胰液以及模拟胆汁在 24 小时孵育期间测定模拟胃肠道条件。将益生菌悬浮液用作对照。用微生物聚合物进行包埋的结果表明，1%黄原胶与 0.75%结冷胶和 1%瓜尔胶与 1%结冷胶的混合物最适合微囊化。pH 值耐受性测试的结果表明，除了在 pH 2.0 下，用黄原胶:结冷胶（1%:0.75%）包埋的乳杆菌的存活时间延长了 6 小时外，与对照相比，细胞的存活率没有改善。在测试的包埋材料中，黄原胶:结冷胶（1%:0.75%）在保护植物乳杆菌和鼠李糖乳杆菌免受模拟胆汁方面最有效，与对照相比，其活菌数提高了 1-2 个对数 CFU。这项研究的结果表明，微生物聚合物是一种有趣的包埋材料来源，应该考虑用于口服递送应用的益生菌包埋的前瞻性研究。
