Pan Xiaoyun, Mao Xin, Cheng Tao, Peng Xiaochun, Zhang Xianlong, Liu Zhongtang, Wang Qi, Chen Yunsu
Department of Orthopaedic Surgery, the Sixth Affiliated People's Hospital, Medical School of ShangHai JiaoTong University, Shanghai, China.
J Surg Res. 2012 Aug;176(2):484-9. doi: 10.1016/j.jss.2011.09.047. Epub 2011 Oct 14.
Local chronic inflammatory reaction plays an important role in the process of aseptic loosening of implants after total joint replacement. In addition, macrophage migration inhibitory factor (MIF) is a key upstream regulator of inflammation, and it is a significant regulator of inflammatory diseases. The purpose of this study is to investigate if the fibroblasts and macrophages in the interfacial membranes overexpress MIF.
The 15 tissue samples of interfacial membranes were obtained from the tissues around the aseptically loosened femoral implants adjacent to osteolytic lesion in 15 patients. The 15 control synovial samples of hip joints were obtained from 15 patients who underwent primary hip arthroplasty because of the fresh fracture of the femoral neck. The levels of MIF protein and mRNA were evaluated by ELISA assay, immunofluorescence labeling, and real-time RT-PCR. Fibroblasts and macrophages were identified by immunofluorescence labeling.
The levels of MIF protein and mRNA were significantly increased, as well as the numbers of MIF+ fibroblasts and macrophages in the interfacial membranes compared with the control synovium.
Not only the macrophages, but also the fibroblasts in interfacial membranes overexpress MIF. MIF may play a significant role in the process of aseptic-loosening implants after total joint replacement.
局部慢性炎症反应在全关节置换术后植入物无菌性松动过程中起重要作用。此外，巨噬细胞移动抑制因子（MIF）是炎症的关键上游调节因子，也是炎症性疾病的重要调节因子。本研究旨在调查界面膜中的成纤维细胞和巨噬细胞是否过度表达MIF。
从15例患者无菌性松动股骨植入物周围靠近溶骨性病变的组织中获取15份界面膜组织样本。从15例因股骨颈新鲜骨折接受初次髋关节置换术的患者中获取15份髋关节对照滑膜样本。通过ELISA检测、免疫荧光标记和实时RT-PCR评估MIF蛋白和mRNA水平。通过免疫荧光标记鉴定成纤维细胞和巨噬细胞。
与对照滑膜相比，界面膜中MIF蛋白和mRNA水平显著升高，MIF+成纤维细胞和巨噬细胞数量也显著增加。
不仅巨噬细胞，界面膜中的成纤维细胞也过度表达MIF。MIF可能在全关节置换术后植入物无菌性松动过程中起重要作用。
