Senomyx Inc., 4767 Nexus Centre Drive, San Diego, CA 92121, United States.
Talanta. 2011 Dec 15;87:307-10. doi: 10.1016/j.talanta.2011.09.063. Epub 2011 Oct 5.
We describe a method for expanding the linear dynamic range for multiple reaction monitoring (MRM) in quantitative liquid chromatography/tandem mass spectrometry (LC-MS/MS) using additional transitions for isotopologues. In addition to the regular transition for the highest possible sensitivity, a transition corresponding to the less abundant isotopologue ions was utilized. This decreases saturation at the ion detector; the sensitivity reduction increases the upper dynamic limit. We demonstrated this for a rat plasma assay for a candidate flavor compound; the linear dynamic range increased by an order of magnitude from 3 to 6,000 ng/mL with the regular MRM alone to 3-60,000 ng/mL using additionally the isotopologue transition.
我们描述了一种使用同位素加和物的额外转换来扩展定量液相色谱/串联质谱（LC-MS/MS）中多重反应监测（MRM）线性动态范围的方法。除了具有最高灵敏度的常规转换之外，还利用了对应于较少丰度同位素离子的转换。这会降低离子检测器的饱和程度；灵敏度的降低会增加上限动态范围。我们在一种用于候选调味化合物的大鼠血浆分析中证明了这一点；单独使用常规 MRM 的线性动态范围从 3 到 6000ng/mL 增加到 3-60000ng/mL，使用同位素加和物的额外转换。
